Spectral decomposition of iron-sulfur clusters
| Autor: | Serge Nader, Sheref S. Mansy, Isaiah O. Betinol |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Iron Biophysics Molecular Conformation chemistry.chemical_element Iron–sulfur cluster 01 natural sciences Biochemistry Spectral line Matrix decomposition 03 medical and health sciences chemistry.chemical_compound Peptide Library Mössbauer spectroscopy Cluster (physics) Ferrous Compounds Spectroscopy Molecular Biology 030304 developmental biology 0303 health sciences 010401 analytical chemistry Electron Spin Resonance Spectroscopy Cell Biology Sulfur 0104 chemical sciences chemistry Atomic electron transition Chemical physics Spectrophotometry Ultraviolet Peptides |
| Zdroj: | Analytical biochemistry. 629 |
| ISSN: | 1096-0309 |
| Popis: | The near universal availability of UV-Visible spectrophotometers makes this instrument a highly exploited tool for the inexpensive, rapid examination of iron-sulfur clusters. Yet, the analysis of iron-sulfur cluster reconstitution experiments by UV-Vis spectroscopy is notoriously difficult due to the presence of broad, ill-defined peaks. Other types of spectroscopies, such as electron paramagnetic resonance spectroscopy and Mossbauer spectroscopy, are superior in characterizing the type of cluster present and their associated electronic transitions but require expensive, less readily available equipment. Here, we describe a tool that utilizes the accessible and convenient platform of Microsoft Excel to allow for the semi-quantitative analysis of iron-sulfur clusters by UV-Vis spectroscopy. This tool, which we call Fit-FeS, could potentially be used to additionally decompose spectra of solutions containing chromophores other than iron-sulfur clusters. |
| Databáze: | OpenAIRE |
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