The Selenophosphate Synthetase Gene fromLeishmania major
| Autor: | P. Cyril Jayakumar, Milind S. Patole, Vijay V. Musande, Yogesh S. Shouche |
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| Rok vydání: | 2004 |
| Předmět: |
Molecular Sequence Data
Protozoan Proteins Biology Biochemistry chemistry.chemical_compound Endocrinology Escherichia coli Genetics Animals Humans Coding region Amino Acid Sequence Molecular Biology Peptide sequence Gene Leishmania major chemistry.chemical_classification Sequence Homology Amino Acid Selenocysteine Phosphotransferases Molecular biology Amino acid Open reading frame genomic DNA Restriction enzyme chemistry Drosophila Sequence Alignment |
| Zdroj: | DNA Sequence. 15:66-70 |
| ISSN: | 1042-5179 |
| Popis: | Selenophosphate synthetase coding sequence was cloned from Leishmania major by RT-PCR amplification. The DNA sequence was found to have an open reading frame encoding protein with 398 amino acids and does not have in-frame UGA codon. The deduced amino acid sequence indicates that it has cysteine residue instead of selenocysteine at the active site of enzyme. Amino acid sequence alignment of Selenophosphate synthetase from parasite with the human enzyme showed approximately 45% homology. The sequences also indicated presence of conserved amino acid residues and motifs that are present in mammalian Selenophosphate synthetase. Southern analysis done with restriction enzyme digested genomic DNA and pulse filed separated chromosome suggests that L. major genome contain a single copy of Selenophosphate synthetase sequence. Expression analysis by Northern analysis and RT-PCR indicated Selenophosphate synthetase mRNA is present in promastigote and amastigote stages of parasite. |
| Databáze: | OpenAIRE |
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