DNA-protein interaction dynamics at the Lamin B2 replication origin

Autor: Fiorenzo A. Peverali, Luca Puzzi, Laura Marchetti, Mauro Giacca, Giuseppe Biamonti
Přispěvatelé: Puzzi, Luca, Marchetti, Laura, Peverali, Fiorenzo A., Biamonti, Giuseppe, Giacca, Mauro
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Proto-Oncogene Proteins c-jun
Origin Recognition Complex
Eukaryotic DNA replication
Pre-replication complex
HeLa Cell
DNA replication origin
Topoisomerase II Inhibitor
Mitochondrial Membrane Transport Proteins
DNA replication factor CDT1
Mitochondrial Precursor Protein Import Complex Proteins
Topoisomerase II Inhibitors
Promoter Regions
Genetic

Genetics
AP-1
Fos
Jun
Origin recognition complex
Transcription factor
Cell Line
Tumor

DNA
DNA Replication
G1 Phase
HeLa Cells
Homeodomain Proteins
Humans
Immunoprecipitation
Lamin Type B
Protein Binding
Proto-Oncogene Proteins c-fos
Replication Origin
Thiobarbiturates
Transcription Factor AP-1
Upstream Stimulatory Factors
Molecular Biology
Developmental Biology
Cell Biology
Tumor
biology
Homeodomain Protein
Fo
Thiobarbiturate
Human
Proto-Oncogene Proteins c-fo
Upstream Stimulatory Factor
Cell Line
Promoter Regions
Control of chromosome duplication
Genetic
DNA replication
Mitochondrial Membrane Transport Protein
Licensing factor
biology.protein
Reports
Popis: To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function.
Databáze: OpenAIRE