DNA-protein interaction dynamics at the Lamin B2 replication origin
Autor: | Fiorenzo A. Peverali, Luca Puzzi, Laura Marchetti, Mauro Giacca, Giuseppe Biamonti |
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Přispěvatelé: | Puzzi, Luca, Marchetti, Laura, Peverali, Fiorenzo A., Biamonti, Giuseppe, Giacca, Mauro |
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Proto-Oncogene Proteins c-jun
Origin Recognition Complex Eukaryotic DNA replication Pre-replication complex HeLa Cell DNA replication origin Topoisomerase II Inhibitor Mitochondrial Membrane Transport Proteins DNA replication factor CDT1 Mitochondrial Precursor Protein Import Complex Proteins Topoisomerase II Inhibitors Promoter Regions Genetic Genetics AP-1 Fos Jun Origin recognition complex Transcription factor Cell Line Tumor DNA DNA Replication G1 Phase HeLa Cells Homeodomain Proteins Humans Immunoprecipitation Lamin Type B Protein Binding Proto-Oncogene Proteins c-fos Replication Origin Thiobarbiturates Transcription Factor AP-1 Upstream Stimulatory Factors Molecular Biology Developmental Biology Cell Biology Tumor biology Homeodomain Protein Fo Thiobarbiturate Human Proto-Oncogene Proteins c-fo Upstream Stimulatory Factor Cell Line Promoter Regions Control of chromosome duplication Genetic DNA replication Mitochondrial Membrane Transport Protein Licensing factor biology.protein Reports |
Popis: | To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function. |
Databáze: | OpenAIRE |
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