Modulating effect of ginseng saponins on heterologously expressed HERG currents in Xenopus oocytes
| Autor: | Jin-Bong Park, Jin-kyu Park, Sook-jin Son, Yong-duk Kim, Hyo-shin Kim, Cuk-Seong Kim, Byeong Hwa Jeon, Kyu-Seung Lee, Kwang-Jin Kim |
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| Rok vydání: | 2005 |
| Předmět: |
ERG1 Potassium Channel
congenital hereditary and neonatal diseases and abnormalities Patch-Clamp Techniques Ginsenosides hERG Xenopus Action Potentials Panax Pharmacology complex mixtures Xenopus laevis Ginseng Animals Pharmacology (medical) cardiovascular diseases Patch clamp biology Chemistry food and beverages General Medicine biology.organism_classification Ether-A-Go-Go Potassium Channels Potassium channel carbohydrates (lipids) Potassium Channels Voltage-Gated Oocytes biology.protein |
| Zdroj: | Acta Pharmacologica Sinica. 26:551-558 |
| ISSN: | 1745-7254 1671-4083 |
| Popis: | To examine the effects of ginseng saponins on the heterologously expressed human ether-a-go-go related gene (HERG) that encodes the rapid component of the delayed rectifier K+ channel.A two-electrode voltage clamp technique was used. HERG currents were recorded in Xenopus oocytes injected with HERG cRNA.Crude saponins of Korean red ginseng (GS) induced a minimal increase of the maximal HERG conductance without changes in the voltage-dependent HERG current activation and inactivation curves. GS, however, decelerated HERG current deactivation in a concentration-dependent manner, which was more noticeable with panaxitriol (PT) than panaxidiol (PD). Consistently, ginseng saponins increased the HERG deactivation time constants with the order of potency of Rg1 (a major component of PT)Rf1Rb1 (a major component of PD). Re had little effect on HERG deactivation. During a cardiac action potential, GS increased the outward HERG current.Ginseng saponins enhance HERG currents, which could be in part a possible mechanism of the shortening cardiac action potential of ginseng saponins. |
| Databáze: | OpenAIRE |
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