Hematological and Immunological plasma assays for grass carp (Ctenopharyngodon idella) infected with Aeromonas hydrophila as an immune model in carp aquaculture
| Autor: | Xiaoyan Xu, Lisen Li, Jiale Li, Yun-Fei Dang, Wen-Ji Huang, Yubang Shen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Carps Lymphocyte Fisheries Immunologic Tests Aquatic Science Hematocrit Microbiology Blood cell Fish Diseases 03 medical and health sciences Immune system medicine Animals Environmental Chemistry Carp Hematologic Tests biology medicine.diagnostic_test Monocyte 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Aeromonas hydrophila Grass carp 030104 developmental biology medicine.anatomical_structure Immunology 040102 fisheries 0401 agriculture forestry and fisheries Gram-Negative Bacterial Infections |
| Zdroj: | Fish & Shellfish Immunology. 55:647-653 |
| ISSN: | 1050-4648 |
| Popis: | Aeromonas hydrophila is the causative agent of bacterial septicemia, a common disease observed in grass carp, Ctenopharyngodon idella. In our study, C. idella specimens were infected with A. hydrophila, and parameters of Hematological and Immunological plasma parameters were monitored. At blood cell level, levels of red blood cells (RBCs), hematocrit (HCT), and mean corpuscular volume (MCV) showed no differences between the treatment and control groups, but levels of white blood cells (WBCs) increased. The monocyte and neutrophil varied significant according to stimulation by A. hydrophila at 1 DPI, the thrombocyte and lymphocyte at 14 and 21 DPI. At serum level, total protein, lysozyme, and IgM increased at the early infection phase and then decreased at other time points; however, peroxidase levels were significantly lower in the treatment group than that in the control group during the early infection phase. ACH50 was significantly higher in the treatment group than that in the control group during the late infection phase. On the basis of the results, we suggest that innate and adaptive immune mechanisms of C. idella are able to neutralize the virulence factors secreted by A. hydrophila. Our findings would help in understanding the mechanisms underlying resistance to infection by A. hydrophila. |
| Databáze: | OpenAIRE |
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