RT-PCR tests for sensitive detection of the major Ranunculus -infecting viruses: field and in vitro applications
Autor: | Sacco E., Borghi, Rabaglio M., Lenzi R., Ciuffo M., Ruffoni B., Vaira A. M. |
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Rok vydání: | 2018 |
Předmět: |
Ranunculus
0106 biological sciences 0301 basic medicine biology plant viruses Plant Science Horticulture Diagnostic tools biology.organism_classification 01 natural sciences Virology diagnostic tools In vitro 03 medical and health sciences 030104 developmental biology Real-time polymerase chain reaction Italy Plant virus Genetics in vitro virus eradication shoot apical meristem culture Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Plant pathology 67 (2018): 1967–1976. doi:10.1111/ppa.12912 info:cnr-pdr/source/autori:Sacco E., Borghi, C., Rabaglio M., Lenzi R., Ciuffo M., Ruffoni B., Vaira A. M./titolo:RT-PCR tests for sensitive detection of the major Ranunculus-infecting viruses: field and in vitro applications/doi:10.1111%2Fppa.12912/rivista:Plant pathology (Print)/anno:2018/pagina_da:1967/pagina_a:1976/intervallo_pagine:1967–1976/volume:67 |
ISSN: | 0032-0862 |
DOI: | 10.1111/ppa.12912 |
Popis: | Buttercup (Ranunculus asiaticus, family Ranunculaceae) hybrids are considered a popular and valuable ornamental crop in Europe and are cultivated both for cut flowers and for pot or border plants. In the Liguria region of northern Italy, Ranunculus hybrid cultivation has dramatically increased over recent years and the related sanitary issues regarding virus infection need to be carefully monitored in order to maintain commercial competitiveness. Presently, ELISA is the most used diagnostic tool for virus diagnosis, but if 'mother plants' with high economic value are to be tested, more sensitive molecular diagnostic tools are needed, together with efficient protocols for in vitro culture to eradicate viruses. In this study, new RT-PCR protocols were tested on virus-infected Ranunculus hybrid plants, indexed for the 13 most important Ranunculus-infecting viruses. Rapid, uniform and effective procedures for molecular diagnosis were devised in order to promote an easy technology transfer from scientific institutions to laboratories of small and medium private enterprises. Moreover, an in vitro culture procedure was established for this species, which proved to be extremely effective in virus eradication. Advanced technology and sustainable quality production of high economic value plants are keys for future competitiveness in floriculture. |
Databáze: | OpenAIRE |
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