Targeting chemo-proton therapy on C6 cell line using superparamagnetic iron oxide nanoparticles conjugated with folate and paclitaxel

Autor:	Seong Hee Kang, Bo Sun Kang, Seong Pyo Hong
Rok vydání:	2018
Předmět:	Radiation-Sensitizing Agents Paclitaxel Superparamagnetic iron oxide nanoparticles Intracellular Space Nanoparticle 02 engineering and technology Conjugated system Brain cancer C6 cells 03 medical and health sciences chemistry.chemical_compound Folic Acid 0302 clinical medicine Cell Line Tumor Proton Therapy Animals Radiology Nuclear Medicine and imaging Magnetite Nanoparticles Proton therapy Drug Carriers Radiological and Ultrasound Technology Brain Neoplasms Biological Transport 021001 nanoscience & nanotechnology Rats chemistry 030220 oncology & carcinogenesis Drug delivery Cancer research Glioblastoma 0210 nano-technology
Zdroj:	International Journal of Radiation Biology. 94:1006-1016
ISSN:	1362-3095 0955-3002
DOI:	10.1080/09553002.2018.1495854
Popis:	This report presents novel nanoparticle-based drug delivery system (NPDDS) aiming to targeting chemo-proton therapy (TCPT) to improve the therapeutic efficacy on brain cancer treatments.A NPDDS, superparamagnetic iron oxide nanoparticles conjugated with folate and paclitaxel, was synthesized and applied to C6 brain cancer cell line that was prepared for TCPT. The characterization of NPDDS was analyzed by transmission electron microscope (TEM) and Fourier transform infrared (FTIR) spectroscopy. The uptake of NPDDS into the cytoplasm of C6 cells was observed by confocal laser scanning microscopy (CLSM). The therapeutic efficacy of proton beam was quantitatively evaluated by flow cytometry and clonogenic assay at various radiation dose.NPDDS was synthesized in the uniform size distribution with a mean diameter of 5.44 ± 0.70 nm, and it showed no significant cytotoxicity at the concentration lower than 200 ng/mL. Radiosensitization enhancement factors of PTX, D-SPIONs and FA-PTX-D-SPIONs were 1.35, 1.16 and 1.52, respectively.It was demonstrated that TCPT improved the therapeutic efficacy of the proton beam therapy when the synthesized novel NPDDS was administrated. The improvement in therapeutic efficacy was achieved by the synergetic effect of drug delivery increased by FA, radiosensitivity increased by PTX and absorption of proton energy increased by SPIONs.
Databáze:	OpenAIRE
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