Enterovirus D68 detection in respiratory specimens: Association with severe disease

Autor: Anny Dewilde, Marie Fatoux, Cécile Henquell, Ilka Engelmann, Enagnon Kazali Alidjinou, Audrey Mirand, Mouna Lazrek, Didier Hober
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), CHU Clermont-Ferrand, Laboratoire de Virologie, Pathogenèse virale du diabète de type 1 - ULR 3610 (Laboratoire de Virologie), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.disease_cause
Bronchoalveolar Lavage
Severity of Illness Index
Disease Outbreaks
0302 clinical medicine
Epidemiology
030212 general & internal medicine
Respiratory system
Child
Lung
Respiratory Tract Infections
Research Articles
ComputingMilieux_MISCELLANEOUS
Aged
80 and over
Enterovirus D
Human
Respiratory distress
sequencing
Middle Aged
3. Good health
Hospitalization
Infectious Diseases
Child
Preschool
Female
France
Research Article
Adult
medicine.medical_specialty
Adolescent
Genotype
respiratory tract infection
Myelitis
Real-Time Polymerase Chain Reaction
[SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology
myelitis
Young Adult
03 medical and health sciences
Virology
Intensive care
Enterovirus Infections
medicine
Humans
Paralysis
Clinical significance
Typing
Aged
business.industry
human enterovirus 68
Infant
medicine.disease
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
030104 developmental biology
Cough
Enterovirus
business
Multiplex Polymerase Chain Reaction
acute flaccid paralysis
Zdroj: Journal of Medical Virology
Journal of Medical Virology, 2017, 89 (7), pp.1201-1207. ⟨10.1002/jmv.24772⟩
Journal of Medical Virology, Wiley-Blackwell, 2017, 89 (7), pp.1201-1207. ⟨10.1002/jmv.24772⟩
ISSN: 0146-6615
1096-9071
DOI: 10.1002/jmv.24772
Popis: Molecular techniques increased the number of documented respiratory infections. In a substantial number of cases the causative agent remains undetected. Since August 2014, an increase in Enterovirus(EV)-D68 infections was reported. We aimed to investigate epidemiology and clinical relevance of EV-D68. From June to December 2014 and from September to December 2015, 803 and 847 respiratory specimens, respectively, were tested for respiratory viruses with a multiplex RT-PCR. This multiplex RT-PCR does not detect EV-D68. Therefore, 457 (2014) and 343 (2015) specimens with negative results were submitted to an EV-specific-RT-PCR. EV-positive specimens were tested with an EV-D68-specific-RT-PCR and genotyped. Eleven specimens of 2014 tested positive in the EV-specific-RT-PCR and of these seven were positive in the EV-D68-specific-RT-PCR. Typing confirmed these as EV-D68. Median age of EV-D68-positive patients was 3 years (1 month-91 years). Common symptoms included fever (n = 6, 86%), respiratory distress (n = 5, 71%), and cough (n = 4, 57%). All EV-D68-positive patients were admitted to hospital, 4 (57%) were admitted to intensive care units and 6 (86%) received oxygen. One patient suffered from acute flaccid paralysis. Seven specimens of 2015 were positive in the EV-specific-RT-PCR but negative in the EV-D68-specific-RT-PCR. In conclusion, use of an EV-specific-RT-PCR allowed us to detect EV-D68 circulation in autumn 2014 that was not detected by the multiplex RT-PCR and was associated with severe disease.
Databáze: OpenAIRE
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