Regulation of Vascular Endothelial Growth Factor Expression by cAMP in Rat Aortic Smooth Muscle Cells
| Autor: | Jean-Baptiste Michel, Maria E. Pueyo, Gisela D'Angelo, Yan Chen |
|---|---|
| Přispěvatelé: | Mécanismes, pathogénies et thérapeutiques expérimentales de remodelage, Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), D'ANGELO, Gisela |
| Rok vydání: | 1998 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Cell type Adenosine Phosphodiesterase Inhibitors Angiogenesis Stimulation Endothelial Growth Factors Biology Muscle Smooth Vascular 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 1-Methyl-3-isobutylxanthine [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Internal medicine Cyclic AMP medicine Animals Iloprost RNA Messenger Aorta 030304 developmental biology Lymphokines 0303 health sciences Forskolin Vascular Endothelial Growth Factors Colforsin Isoproterenol Cobalt Cell Biology Fibroblasts Rats Enzyme Activation Vascular endothelial growth factor Endothelial stem cell Vascular endothelial growth factor B Endocrinology Gene Expression Regulation chemistry 030220 oncology & carcinogenesis [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Endothelium Vascular Adenylyl Cyclases medicine.drug |
| Zdroj: | Experimental Cell Research Experimental Cell Research, Elsevier, 1998, 238 (2), pp.354-358. ⟨10.1006/excr.1997.3864⟩ Experimental Cell Research, 1998, 238 (2), pp.354-358. ⟨10.1006/excr.1997.3864⟩ |
| ISSN: | 0014-4827 1090-2422 |
| DOI: | 10.1006/excr.1997.3864 |
| Popis: | International audience; Vascular endothelial growth factor (VEGF) is an endothelial cell mitogen which stimulates angiogenesis. VEGF is regulated by multiple factors such as hypoxia, phorbol esters, and growth factors. However, data concerning the expression of VEGF in the different vascular cell types and its regulation by cAMP are not available. In the present study, we have investigated the effect of adenylate cyclase activation on VEGF mRNA expression in rat vascular cells in primary culture. Basal VEGF expression is greater in smooth muscle cells than in endothelial cells and fibroblasts. A 4-h treatment with forskolin (10−5M) induced a 2-fold stimulation of VEGF mRNA expression in smooth muscle cells and fibroblasts, but, in contrast, did not affect VEGF expression in endothelial cells. In smooth muscle cells, a pharmacologically induced increase in intracellular cAMP levels using iloprost or isoprenaline led to a rise in VEGF mRNA expression comparable to that induced by forskolin. Adenosine, which increases cAMP levels in smooth muscle cells, also increases VEGF expression. Moreover, the 2.2-fold stimulation of VEGF expression by adenosine was enhanced following a cotreatment with cobalt chloride (a hypoxia miming agent). The observed additive effect (4.3-fold increase) suggests that these two factors, hypoxia and adenosine, regulate VEGF mRNA expression in smooth muscle cells by independent mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect