Overstretching Double-Stranded RNA, Double-Stranded DNA, and RNA-DNA Duplexes
| Autor: | Ulrich Bockelmann, Lena Melkonyan, Thierry Bizebard, Mathilde Bercy |
|---|---|
| Přispěvatelé: | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris-Sciences-et-Lettres (https://www.psl.eu/), Institut de biologie physico-chimique (IBPC) |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Biophysics RNA DNA Articles Double stranded rna Biomechanical Phenomena 03 medical and health sciences chemistry.chemical_compound RNA silencing 0302 clinical medicine chemistry Optical tweezers Helix Nucleic Acid Conformation Biological cell Salts Double stranded ComputingMilieux_MISCELLANEOUS 030217 neurology & neurosurgery RNA Double-Stranded 030304 developmental biology |
| Zdroj: | Biophysical Journal Biophysical Journal, Biophysical Society, 2019, 117 (3), pp.509-519. ⟨10.1016/j.bpj.2019.07.003⟩ Biophys J |
| ISSN: | 0006-3495 1542-0086 |
| DOI: | 10.1016/j.bpj.2019.07.003 |
| Popis: | Using single-molecule force measurements, we compare the overstretching transition of the four types of duplexes composed of DNA or RNA strands. Three of the four extremities of each double helix are attached to two microscopic beads, and a stretching force is applied with a dual-beam optical trapping interferometer. We find that overstretching occurs for all four duplexes with small differences between the plateau forces. Double-stranded RNA (dsRNA) exhibits a smooth transition in contrast to the other three duplexes that show sawtooth patterns, the latter being a characteristic signature of peeling. This difference is observed for a wide range of experimental conditions. We present a theoretical description that explains the difference and predicts that peeling and bubble formation do not occur in overstretching double-stranded RNA. Formation of S-RNA is proposed, an overstretching mechanism that contrary to the other two does not generate single strands. We suggest that this singular RNA property helps RNA structures to assemble and play their essential roles in the biological cell. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|