Isolation from a sheep of an attaching and effacing Escherichia coli O115:H− with a novel combination of virulence factors
| Autor: | John M. Roe, Adrian L. Cookson, Geoffrey R. Pearson, Andrew Wales, Christine M Hayes, Martin J. Woodward |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Genotype Colon Bacterial Toxins Sheep Diseases Virulence medicine.disease_cause Polymerase Chain Reaction Microbiology Bacterial Adhesion Plasmid Culture Techniques Escherichia coli medicine Animals Serotyping Adhesins Bacterial Escherichia coli Infections Intimin Antiserum Sheep biology Escherichia coli Proteins Rectum General Medicine biology.organism_classification Enterobacteriaceae Microscopy Electron Microscopy Electron Scanning Vero cell Carrier Proteins Bacteria Plasmids |
| Zdroj: | Journal of Medical Microbiology. 51:1041-1049 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Attaching and effacing (AE) lesions were observed in the caecum, proximal colon and rectum of one of four lambs experimentally inoculated at 6 weeks of age with Escherichia coli O157:H7. However, the attached bacteria did not immunostain with O157-specific antiserum. Subsequent bacteriological analysis of samples from this animal yielded two E. coli O115:H(-) strains, one from the colon (CO) and one from the rectum (RC), and those bacteria forming the AE lesions were shown to be of the O115 serogroup by immunostaining. The O115:H(-)isolates formed microcolonies and attaching and effacing lesions, as demonstrated by the fluorescence actin staining test, on HEp-2 tissue culture cells. Both isolates were confirmed by PCR to encode the epsilon (epsilon) subtype of intimin. Supernates of both O115:H(-) isolates induced cytopathic effects on Vero cell monolayers, and PCR analysis verified that both isolates encoded EAST1, CNF1 and CNF2 toxins but not Shiga-like toxins. Both isolates harboured similar sized plasmids but PCR analysis indicated that only one of the O115:H(-) isolates (CO) possessed the plasmid-associated virulence determinants ehxA and etpD. Neither strain possessed the espP, katP or bfpA plasmid-associated virulence determinants. These E. coli O115:H(-) strains exhibited a novel combination of virulence determinants and are the first isolates found to possess both CNF1 and CNF2. |
| Databáze: | OpenAIRE |
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