Is UV-Induced Electron-Driven Proton Transfer Active in a Chemically Modified A•T DNA Base Pair?
| Autor: | Arsène F. M. Chemin, Hugo J. B. Marroux, M. Carmen Galan, Thomas A. A. Oliver, Andrew J. Orr-Ewing, Steven T. G. Street, Gareth M. Roberts, Katharina Röttger, Alexander S. Henderson, Emma Elsdon |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Proton
Ultraviolet Rays Base pair Analytical chemistry Electrons 010402 general chemistry 01 natural sciences chemistry.chemical_compound BCS and TECS CDTs Ultrafast laser spectroscopy Materials Chemistry Physical and Theoretical Chemistry Base Pairing Chloroform 010405 organic chemistry Adenine Relaxation (NMR) DNA 0104 chemical sciences Surfaces Coatings and Films Crystallography Monomer chemistry Yield (chemistry) Quantum Theory Protons Thymine Derivative (chemistry) |
| Zdroj: | Roettger, K, Marroux, H, Chemin, A, Elsdon, E, Oliver, T A A, Street, S, Henderson, A S J, Galan, C, Orr-Ewing, A & Roberts, G 2017, ' Is UV-Induced Electron-Driven Proton Transfer Active in a Chemically Modified A•T DNA Base Pair? ', Journal of Physical Chemistry B, vol. 121, no. 17, pp. 4448-4455 . https://doi.org/10.1021/acs.jpcb.7b02679 The Journal of Physical Chemistry B |
| Popis: | Transient electronic and vibrational absorption spectroscopies have been used to investigate whether UV-induced electron-driven proton transfer (EDPT) mechanisms are active in a chemically modified adenine-thymine (A·T) DNA base pair. To enhance the fraction of biologically relevant Watson-Crick (WC) hydrogen-bonding motifs and eliminate undesired Hoogsteen structures, a chemically modified derivative of A was synthesized, 8-(tert-butyl)-9-ethyladenine (8tBA). Equimolar solutions of 8tBA and silyl-protected T nucleosides in chloroform yield a mixture of WC pairs, reverse WC pairs, and residual monomers. Unlike previous transient absorption studies of WC guanine-cytosine (G·C) pairs, no clear spectroscopic or kinetic evidence was identified for the participation of EDPT in the excited-state relaxation dynamics of 8tBA·T pairs, although ultrafast (sub-100 fs) EDPT cannot be discounted. Monomer-like dynamics are proposed to dominate in 8tBA·T. |
| Databáze: | OpenAIRE |
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