Histochemical markers reveal an unexpected heterogeneous composition of the renal embryonic collecting duct epithelium
| Autor: | Ernst Brandt, Will W. Minuth, Sabine Kloth, Roland Moll, Joachim Aigner |
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| Rok vydání: | 1993 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Peanut agglutinin Pathology medicine.medical_specialty Arachis Molecular Sequence Data Antigens CD7 Nephron Biology Epithelium Cholangiocyte Peanut Agglutinin Cytokeratin Antigens CD Lectins medicine Animals Intercalated Cell Amino Acid Sequence Kidney Tubules Collecting Carbonic Anhydrases Histocytochemistry Transdifferentiation Antibodies Monoclonal medicine.anatomical_structure Animals Newborn Nephrology Microscopy Electron Scanning biology.protein Interleukin-2 Keratins Rabbits Plant Lectins Duct (anatomy) Biomarkers |
| Zdroj: | Kidney International. 44:527-536 |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.1993.277 |
| Popis: | Histochemical markers reveal an unexpected heterogeneous composition of the renal embryonic collecting duct epithelium. The ampullary collecting duct epithelium acts as an inductor in the embryonic and neonatal kidney. It induces the formation of all nephron generations and thus determines the whole architecture of the kidney. As the organ matures, the collecting duct epithelium itself transdifferentiates. The ampullary inductor epithelium, which appears homogeneous as revealed by light microscopy, develops into the well-known heterogeneous epithelium of the mature collecting duct consisting of light principal and dark intercalated cells. Up to now the mechanisms initiating and regulating this transdifferentiation step are unknown. Only very few data are available concerning functional characteristics of the ampullary epithelial cells of neonatal rabbit kidney. Therefore, a characterization of the collecting duct ampullary cells was carried out by means of immunohistological techniques using a set of different monoclonal antibodies and the lectin peanut agglutinin. All epithelial cells within the ampullary tip and neck were positive for cytokeratin 19, an intermediate filament protein. On the other hand, the monoclonal antibody CD 7 revealed a clear cut boundary between the ampullary neck and the ampullary tip region. Furthermore, after incubation with the monoclonal antibody BO-7 specifically reacting with intercalated cells of the mature collecting duct, both labeled and unlabeled cells were observed within the whole ampullary epithelium. These results were confirmed by scanning electron microscopical investigations which revealed two distinct epithelial cell populations. Thus, an unexpected heterogeneity of the ampullary epithelium could be demonstrated. |
| Databáze: | OpenAIRE |
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