Evidence of hexaploid karyotype in shortnose sturgeon
| Autor: | F. Fontana, Joseph M. Quattro, Vincent A. MudrakV.A. Mudrak, Leonardo Congiu, Serge I. DoroshovS.I. Doroshov, Kent WareK. Ware, Theodore J. I. Smith |
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| Rok vydání: | 2008 |
| Předmět: |
Acipenseriformes
Satellite DNA sturgeon phylogeny satellite DNA Mitosis Genome DNA Ribosomal Polyploidy Nucleic acid thermodynamics Gene Duplication Gene duplication Genetics Acipenser Animals Molecular Biology biology 5S rDNA allohexaploidy Fishes Nucleic Acid Hybridization Karyotype General Medicine biology.organism_classification Shortnose sturgeon Karyotyping Biotechnology |
| Zdroj: | Genome. 51(2) |
| ISSN: | 0831-2796 |
| Popis: | A karyotype analysis by several staining techniques was carried out on triplicate samples of the shortnose sturgeon, Acipenser brevirostrum . The chromosome number was found to be 2n = 372 ± 6. A representative karyotype of 374 chromosomes was composed of 178 metacentrics/submetacentrics and 196 telocentrics/acrocentrics and microchromosomes. The signals of fluorescent in situ hybridization (FISH) with a HindIII satellite DNA probe were visible on 14 chromosomes. The signals obtained with a PstI satellite DNA probe appeared on 12 chromosomes. The FISH with a 5S rDNA probe revealed fluorescent signals on 6 chromosomes. These last results, compared with 2 signals in species with about 120 chromosomes and 4 in species with 240, support the hypothesis that A. brevirostrum is a hexaploid species, probably of hybrid origin. Based on these results, we propose a model explaining speciation events occurring in sturgeons by hybridization, genome duplication, and diploidization. |
| Databáze: | OpenAIRE |
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