Quantitation of Compounds in Wine Using 1H NMR Spectroscopy: Description of the Method and Collaborative Study
| Autor: | Claus-Dieter Patz, Christian Kost, Reinhard Ristow, Helmut Wachter, Rolf Godelmann |
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| Rok vydání: | 2016 |
| Předmět: |
Preservative
Proton Magnetic Resonance Spectroscopy Malates Analytical chemistry Shikimic Acid Wine Acetates 01 natural sciences Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Fumarates Environmental Chemistry Spectroscopy Pharmacology Chromatography 010401 analytical chemistry food and beverages 04 agricultural and veterinary sciences 040401 food science Sorbic Acid Quantitative determination 0104 chemical sciences Glucose chemistry Proton NMR Laboratories Sorbic acid Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 99:1295-1304 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.15-0318 |
| Popis: | To examine whether NMR analysis is a suitable method for the quantitative determination of wine components, an international collaborative trial was organized to evaluate the method according to the international regulations and guidelines of the German Institute for Standardization/International Organization for Standardization, AOAC INTERNATIONAL, the International Union of Pure and Applied Chemistry, and the International Organization of Vine and Wine. Sugars such as glucose; acids such as malic, acetic, fumaric, and shikimic acids (the latter two as minor components); and sorbic acid, a preservative, were selected for the exemplary quantitative determination of substances in wine. Selection criteria for the examination of sample material included different NMR spectral signal types (singlet and multiplet), as well as the suitability of the proposed substances for manual integration at different levels of challenge (e.g., interference as a result of the necessary suppression of a water signal or the coverage of different typical wine concentration ranges for a selection of major components, minor components, and additives). To show that this method can be universally applied, NMR measurement and the method of evaluation were not strictly elucidated. Fifteen international laboratories participated in the collaborative trial and determined six parameters in 10 samples. The values, in particular the reproducibility SD (SR), were compared with the expected Horwitz SD (SH) by forming the quotient SR/SH (i.e., the HorRat value). The resulting HorRat values of most parameters were predominantly between 0.6 and 1.5, and thus of an acceptable range. |
| Databáze: | OpenAIRE |
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