The effect of hydrazide linkers on hyaluronan hydrazone hydrogels
| Autor: | Lenka Hejlová, Petra Šedová, Vladimír Velebný, Matěj Šimek, Radovan Buffa, Hana Vágnerová, Ivana Basarabová, Lenka Kovářová, Jiří Bednařík, Ivana Ščigalková, Peter Šilhár |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Polymers and Plastics
Acylation Hydrazone Biocompatible Materials macromolecular substances 02 engineering and technology 010402 general chemistry Hydrazide complex mixtures 01 natural sciences Chemical kinetics Mice chemistry.chemical_compound Elastic Modulus Polymer chemistry Materials Chemistry Animals Hyaluronic Acid Cytotoxicity Swiss 3T3 Cells chemistry.chemical_classification Schiff base Organic Chemistry Hydrazones technology industry and agriculture Hydrogels Nuclear magnetic resonance spectroscopy Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology 0104 chemical sciences Kinetics Cross-Linking Reagents Hydrazines chemistry Self-healing hydrogels 0210 nano-technology |
| Zdroj: | Carbohydrate Polymers. 216:63-71 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2019.04.011 |
| Popis: | The effect of hydrazide linkers on the formation and mechanical properties of hyaluronan hydrogels was intensively evaluated. The reaction kinetics of hydrazone formation was monitored by NMR spectroscopy under physiological conditions where polyaldehyde hyaluronan (unsaturated: ΔHA-CHO, saturated: HA-CHO) was reacted with various hydrazides to form hydrogels. Linear (adipic, oxalic dihydrazide) and branched (N,N´,N´´-tris(hexanoylhydrazide-6-yl)phosphoric triamide and 4-arm-PEG hydrazide) hydrazides were compared as crosslinking agents. The mechanical properties of hydrogels were also modified by attaching a hydrophobic chain to HA-CHO; however, it was found that this modification did not lead to an increase in hydrogel stiffness. Cytotoxicity tests showed that all tested hydrazide crosslinkers reduced the viability of cells only slightly, and that the final hyaluronan hydrogels were non-toxic materials. |
| Databáze: | OpenAIRE |
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