The non-canonical Wnt-PCP pathway shapes the mouse caudal neural plate
| Autor: | Timothy F. Plageman, Javier Márquez-Rivas, Lance A. Davidson, Dawn Savery, Deepthi S. Vijayraghavan, Beatriz López-Escobar, Roberto Moreno, José Antonio Sánchez-Alcázar, José Manuel Caro-Vega, Patricia Ybot-Gonzalez |
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| Přispěvatelé: | Instituto de Salud Carlos III, Junta de Andalucía, National Institutes of Health (US), National Science Foundation (US), National Institute of Biomedical Imaging and Bioengineering (US) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Neural Tube Contraction (grammar) Cellular differentiation Cell NTDs Nerve Tissue Proteins Biology Apical contraction Caudal neurulation 03 medical and health sciences Mice 0302 clinical medicine Neural Stem Cells Embryonic Structure medicine Animals Wnt-PCP Progenitor cell Molecular Biology NMPs Spinal Dysraphism Wnt Signaling Pathway Non canonical wnt Neural Plate Neural tube Cell Differentiation Mice Mutant Strains Cell biology 030104 developmental biology medicine.anatomical_structure Tissue folding Neural plate 030217 neurology & neurosurgery Developmental Biology Research Article |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) |
| Popis: | The last stage of neural tube (NT) formation involves closure of the caudal neural plate (NP), an embryonic structure formed by neuromesodermal progenitors and newly differentiated cells that becomes incorporated into the NT. Here, we show in mouse that, as cell specification progresses, neuromesodermal progenitors and their progeny undergo significant changes in shape prior to their incorporation into the NT. The caudo-rostral progression towards differentiation is coupled to a gradual reliance on a unique combination of complex mechanisms that drive tissue folding, involving pulses of apical actomyosin contraction and planar polarised cell rearrangements, all of which are regulated by the Wnt-PCP pathway. Indeed, when this pathway is disrupted, either chemically or genetically, the polarisation and morphology of cells within the entire caudal NP is disturbed, producing delays in NT closure. The most severe disruptions of this pathway prevent caudal NT closure and result in spina bifida. In addition, a decrease in Vangl2 gene dosage also appears to promote more rapid progression towards a neural fate, but not the specification of more neural cells. This work was supported by grants from the Instituto de Salud Carlos III (PS09/00050, CP08/00111, CPII14/00033, PI14/01075 and PI17/00693 to P.Y.-G.) co-financed by the European Regional Development Fund “A way to achieve Europe”; the Andalusian Health Service, Junta de Andalucia (Servicio Andaluz de Salud, Junta de Andalucia; PI-0438-2010 to P.Y.-G.); the Andalusian Regional Ministry of Economy, Science and Innovation (Consejerıa de Econom ́ ıa, Innovacio ́ ́ń, Ciencia y Empleo, Junta de Andalucıá ́; P11-cts-7634 to P.Y.-G.); the National Institutes of Health (R01 HD044750 to L.A.D.); and the National Science Foundation (CMMI-1100515 to L.A.D.). In addition, D.S.V. was supported by the “Biomechanics in Regeneration” Training Program from the National Institute of Biomedical Imaging and Bioengineering (BiRM T32 EB003392). |
| Databáze: | OpenAIRE |
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