The Dose of Intravenously Transplanted Bone Marrow Stromal Cells Determines the Therapeutic Effect on Vascular Remodeling in a Rat Model of Ischemic Stroke
| Autor: | Pingyi Xu, Qianmin Yao, Gongxiong Wu, Zijing Liang, Yubao Xie, Junmin Lin, Bin He, Shaoming Li, Zhihua Yang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A medicine.medical_specialty Pathology Basic fibroblast growth factor Blotting Western Biomedical Engineering Ischemia lcsh:Medicine Enzyme-Linked Immunosorbent Assay Vascular Remodeling Mesenchymal Stem Cell Transplantation Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Stroke Transplantation business.industry Cerebral infarction Mesenchymal stem cell lcsh:R Brain Mesenchymal Stem Cells Cell Biology Cerebral Infarction medicine.disease Surgery Rats Vascular endothelial growth factor Vascular endothelial growth factor A Disease Models Animal 030104 developmental biology chemistry Microscopy Fluorescence Fibroblast Growth Factor 2 business 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 25 (2016) |
| ISSN: | 1555-3892 |
| Popis: | The therapeutic benefits of bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation for ischemic stroke have been extensively demonstrated. However, studies on the optimal cell dose for intravenous administration are still limited. This study aimed to determine an appropriate cell dose for BM-MSC intravenous transplantation and to investigate the effect of cell dose on vascular remodeling in a rat model of ischemic stroke. BM-MSCs at doses of 5 × 10 4 (low-dose group), 5 × 10 5 (medium-dose group), and 2 × 10 6 (high-dose group) were intravenously injected into rats at 72 h after ischemia. The therapeutic efficacy of BM-MSCs was evaluated by measuring infarct volume, vascular diameters, capillary area in the peri-infarct zone, level of basic fibroblast growth factor (bFGF) in the peri-infarct zone, and serum vascular endothelial growth factor (VEGF) level at 7 days after ischemia. Compared with the low-dose and control groups, medium-dose and high-dose BM-MSC transplantation significantly reduced the volume of the infarct area, enlarged the diameters of pial vessels and the basilar artery, and increased the capillary area in the peri-infarct zone of the cerebral cortex. Furthermore, transplanted BM-MSCs elevated the expressions of bFGF in the peri-infarct zone and the serum VEGF level. Administration of 5 × 10 5 BM-MSCs is an appropriate cell dose for ischemic stroke therapy in rats. These findings may be helpful for designing future clinical trials. |
| Databáze: | OpenAIRE |
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