Distinct cerebellar engrams in short-term and long-term motor learning
| Autor: | Fumihiro Shutoh, Yun-Qing Li, Etsuko Tarusawa, Soichi Nagao, Wajeeha Aziz, Kazuhiko Nakadate, Andrea Lorincz, Ryuichi Shigemoto, Miwako Masugi-Tokita, Wen Wang, Yugo Fukazawa, Sebnem Kesaf, Elek Molnár |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Time Factors AMPA receptor Synaptic Transmission Synapse Mice Purkinje Cells Nerve Fibers Memory Cerebellum Animals Freeze Fracturing Learning Receptors AMPA Motor Neurons Neuronal Plasticity Multidisciplinary Behavior Animal Long-Term Synaptic Depression musculoskeletal neural and ocular physiology Glutamate receptor Optokinetic reflex Memory Short-Term PNAS Plus nervous system Synapses Synaptic plasticity Excitatory postsynaptic potential Memory consolidation Motor learning Psychology Neuroscience |
| Zdroj: | Proceedings of the National Academy of Sciences. 111 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Cerebellar motor learning is suggested to be caused by long-term plasticity of excitatory parallel fiber-Purkinje cell (PF-PC) synapses associated with changes in the number of synaptic AMPA-type glutamate receptors (AMPARs). However, whether the AMPARs decrease or increase in individual PF-PC synapses occurs in physiological motor learning and accounts for memory that lasts over days remains elusive. We combined quantitative SDS-digested freeze-fracture replica labeling for AMPAR and physical dissector electron microscopy with a simple model of cerebellar motor learning, adaptation of horizontal optokinetic response (HOKR) in mouse. After 1-h training of HOKR, short-term adaptation (STA) was accompanied with transient decrease in AMPARs by 28% in target PF-PC synapses. STA was well correlated with AMPAR decrease in individual animals and both STA and AMPAR decrease recovered to basal levels within 24 h. Surprisingly, long-term adaptation (LTA) after five consecutive daily trainings of 1-h HOKR did not alter the number of AMPARs in PF-PC synapses but caused gradual and persistent synapse elimination by 45%, with corresponding PC spine loss by the fifth training day. Furthermore, recovery of LTA after 2 wk was well correlated with increase of PF-PC synapses to the control level. Our findings indicate that the AMPARs decrease in PF-PC synapses and the elimination of these synapses are in vivo engrams in short- and long-term motor learning, respectively, showing a unique type of synaptic plasticity that may contribute to memory consolidation. |
| Databáze: | OpenAIRE |
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