Potential of label-free detection in high-content-screening applications
| Autor: | Guenter Gauglitz, Martin Mehlmann, Bernd Moehrle, Lutz Steinle, Florian Pröll, Guenther Proll, Michael Kumpf |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Electrophoresis
Bioanalysis Chemistry business.industry High-throughput screening Spectrum Analysis Organic Chemistry Temperature Nanotechnology General Medicine Biochemistry Automation Sensitivity and Specificity Analytical Chemistry Reflectometric interference spectroscopy High-content screening Drug Design Surface plasmon resonance business Biosensor Throughput (business) |
| Zdroj: | Journal of chromatography. A. 1161(1-2) |
| ISSN: | 0021-9673 |
| Popis: | The classical approach of high-content screening (HCS) is based on multiplexed, functional cell-based screening and combines several analytical technologies that have been used before separately to achieve a better level of automation (scale-up) and higher throughput. New HCS methods will help to overcome the bottlenecks, e.g. in the present development chain for lead structures for the pharmaceutical industry or during the identification and validation process of new biomarkers. In addition, there is a strong need in analytical and bioanalytical chemistry for functional high-content assays which can be provided by different hyphenated techniques. This review discusses the potential of a label-free optical biosensor based on reflectometric interference spectroscopy (RIfS) as a bridging technology for different HCS approaches. Technical requirements of RIfS are critically assessed by means of selected applications and compared to the performance characteristics of surface plasmon resonance (SPR) which is currently the leading technology in the area of label-free optical biosensors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect