Efficient mucosal vaccination of a novel classical swine fever virus E2-Fc fusion protein mediated by neonatal Fc receptor
| Autor: | Shaohua Sun, Jianglong Li, Zhenxiang Rong, Xiangmin Li, Hui Ma, Huawei Zhang, Huanchun Chen, Kui Fang, Genxi Hao, Xinxin Li, Ping Qian, Xujiao Ren, Zekai Zhao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Swine
animal diseases 030231 tropical medicine Fc receptor Receptors Fc Antibodies Viral Virus Classical Swine Fever 03 medical and health sciences 0302 clinical medicine Neonatal Fc receptor Immune system Viral Envelope Proteins Animals 030212 general & internal medicine General Veterinary General Immunology and Microbiology biology Histocompatibility Antigens Class I Vaccination Public Health Environmental and Occupational Health Viral Vaccines biology.organism_classification Fusion protein Virology Infectious Diseases Immunization Classical Swine Fever Virus Classical swine fever biology.protein Molecular Medicine Cytokine secretion |
| Zdroj: | Vaccine. 38:4574-4583 |
| ISSN: | 0264-410X |
| Popis: | Classical swine fever (CSF) remains one of the most important highly contagious and fatal viral disease of swine with high morbidity and mortality. CSF is caused by classical swine fever virus (CSFV), a small, enveloped RNA virus of the genus Pestivirus. The aim of this study was to construct the a novel CSFV Fc-fusion recombinant protein and evaluate the efficacy as a vaccine against CSFV. Here, we obtained a novel subunit vaccine expressing CSFV E2 recombinant fusion protein in CHO-S cells. Functional analysis revealed that CSFV Fc-fusion recombinant protein (CSFV-E2-Fc) could bind to FcγRI on antigen-presenting cells (APCs) and significantly increase IgA levels in serum and feces, inducing stronger mucosal immune response in swine. Additionally, CSFV-E2-Fc immunization enhanced CSFV-specific T cell immune response with a Th1-like pattern of cytokine secretion, remarkably stimulated the Th1-biased cellular immune response and humoral immune response. Further, the protective effects of CSFV-E2-Fc subunit vaccines were confirmed. The data suggest that CSFV E2-Fc recombinant fusion protein may be a promising candidate subunit vaccine to elicit immune response and protect against CSFV. |
| Databáze: | OpenAIRE |
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