Fluorescence detection of protein content in house dust: the possible role of keratin
| Autor: | Evgeny A. Shirshin, Olga V. Voloshina, Maxim E. Darvin, Juergen Lademann, Victor V. Fadeev |
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| Rok vydání: | 2015 |
| Předmět: |
Environmental Engineering
Ultrafiltration Analytical chemistry 010501 environmental sciences 01 natural sciences Fluorescence 010309 optics symbols.namesake Lowry protein assay 0103 physical sciences Keratin 0105 earth and related environmental sciences chemistry.chemical_classification Public Health Environmental and Occupational Health Substrate (chemistry) Dust Building and Construction Spectrometry Fluorescence chemistry Excited state Air Pollution Indoor symbols Keratins Raman spectroscopy Raman scattering Environmental Monitoring |
| Zdroj: | Indoor air. 27(2) |
| ISSN: | 1600-0668 |
| Popis: | We propose a fluorescence method for protein content assessment in fine house dust, which can be used as an indicator of the hygienic state of occupied rooms. The results of the measurements performed with 30 house dust samples, including ultrafiltration experiments, strongly suggest that the fluorescence emission of house dust extracts excited at 350 nm is mainly due to protein fragments, which are presumably keratin hydrolysates. This suggestion is supported by several facts: (i) Spectral band shapes for all the samples under investigation are close and correspond to that of keratin; (ii) fluorescence intensity correlates with the total protein content as provided by Lowry assay; (iii) treatment of the samples with proteinase K, which induces keratin hydrolysis, results in fluorescence enhancement without changing fluorescence band shape; and (iv) Raman spectra of keratin and fine house dust samples exhibit a very similar structure. Based on the obtained results and literature data, we propose a hypothesis that keratin is a major substrate for fluorescence species in fine house dust, which are responsible for emission at 350-nm excitation. |
| Databáze: | OpenAIRE |
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