Purification, Preliminary Characterization and Hepatoprotective Effects of Polysaccharides from Dandelion Root
| Autor: | Libiao Luan, Dongwei Wan, Fanglian Yi, Liangliang Cai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Arabinose Taraxacum polysaccharides Pharmaceutical Science 02 engineering and technology Nrf2-Keap1 Plant Roots Analytical Chemistry chemistry.chemical_compound hepatoprotective effects Drug Discovery chemistry.chemical_classification Liver injury Mice Inbred ICR Hexuronic Acids Monosaccharides 021001 nanoscience & nanotechnology Biochemistry Chemistry (miscellaneous) Molecular Medicine Chemical and Drug Induced Liver Injury 0210 nano-technology medicine.drug dandelion root purification endocrine system Rhamnose Dandelion Polysaccharide Article lcsh:QD241-441 Structure-Activity Relationship 03 medical and health sciences lcsh:Organic chemistry Galacturonic acid medicine Animals Humans Physical and Theoretical Chemistry Acetaminophen Plant Extracts Organic Chemistry medicine.disease 030104 developmental biology chemistry Galactose |
| Zdroj: | Molecules, Vol 22, Iss 9, p 1409 (2017) Molecules; Volume 22; Issue 9; Pages: 1409 Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules22091409 |
| Popis: | In this study, purification, preliminary characterization and hepatoprotective effects of water-soluble polysaccharides from dandelion root (DRP) were investigated. Two polysaccharides, DRP1 and DRP2, were isolated from DRP. The two polysaccharides were α-type polysaccharides and didn’t contain protein. DRP1, with a molecular weight of 5695 Da, was composed of glucose, galactose and arabinose, whereas DRP2, with molecular weight of 8882 Da, was composed of rhamnose, galacturonic acid, glucose, galactose and arabinose. The backbone of DRP1 was mainly composed of (1→6)-linked-α-d-Glc and (1→3,4)-linked-α-d-Glc. DRP2 was mainly composed of (1→)-linked-α-d-Ara and (1→)-linked-α-d-Glc. A proof-of-concept study was performed to assess the therapeutic potential of DRP1 and DRP2 in a mouse model that mimics acetaminophen (APAP) -induced liver injury (AILI) in humans. The present study shows DRP1 and DRP2 could protect the liver from APAP-induced hepatic injury by activating the Nrf2-Keap1 pathway. These conclusions demonstrate that the DRP1 and DRP2 might be suitable as functional foods and natural drugs in preventing APAP-induced liver injury. |
| Databáze: | OpenAIRE |
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