Fractionation of neurons and satellite cells from human sensory ganglia in order to study herpesvirus latency
| Autor: | Lewis I. Pizer, Guang-Yun Cai, Myron J. Levin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Cell type Herpesvirus 2 Human viruses Cell Separation Genome Viral Herpesvirus 1 Human HSL and HSV Biology medicine.disease_cause Herpesviridae Trigeminal ganglion Virology Virus latency medicine Humans Aged Neurons Middle Aged medicine.disease biology.organism_classification Molecular biology Virus Latency medicine.anatomical_structure Herpes simplex virus Trigeminal Ganglion DNA Viral Female Satellite (biology) Neuron |
| Zdroj: | Journal of Virological Methods. 104:21-32 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/s0166-0934(02)00032-0 |
| Popis: | A method is described for fractionating human trigeminal ganglia into highly purified populations of neurons and satellite cells in order to study alpha-herpesvirus latency. The method was validated by microscopy of the separated populations and by the observation that only the neuronal population, not the satellite cells, contained herpes simplex virus (HSV) DNA. The frequency of detecting HSV in neurons from ganglia was 3% (43 of 1440 neurons). HSV DNA was not detected in approximately 17,500 satellite cells. The HSV DNA genome copy number in single cells ranged from 2 to 50. These data on the frequency and cellular location of latent HSV indicate that our mechanical fractionation of cell types results in low levels of cross-contamination and provides samples from which cells infected latently can be studied at the single cell level. |
| Databáze: | OpenAIRE |
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