Viromes in Xylariaceae fungi infecting avocado in Spain
| Autor: | Isabel Arjona-Girona, Leonardo Velasco, C. J. López-Herrera, Enrico Cretazzo |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Instituto de Investigación y Formación Agraria y Pesquera (España) |
| Rok vydání: | 2019 |
| Předmět: |
Bunyaviridae
Hypoviridae Entoleuca Ourmia-like virus Genome Viral Rosellinia necatrix Fungal Viruses Plant Roots Tymoviridae Trees Microbiology 03 medical and health sciences Pathosystem Mycoviruses Virology Human virome Amino Acid Sequence Xylariaceae Multiple virus infections Phylogeny RNA Double-Stranded 030304 developmental biology Gammaflexivirus 0303 health sciences Base Sequence Mycelium Sequence Homology Amino Acid Xylariales biology Persea 030302 biochemistry & molecular biology Horizontal virus transmission High-Throughput Nucleotide Sequencing Endornavirus Partitiviridae biology.organism_classification Spain Negative single-stranded RNA virus Mycovirus Nucleic Acid Conformation RNA Viral Tymovirales Metagenomics Sequence Alignment |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0042-6822 |
| Popis: | Four isolates of Entoleuca sp., family Xylariaceae, Ascomycota, recovered from avocado rhizosphere in Spain were analyzed for mycoviruses presence. For that, the dsRNAs from the mycelia were extracted and subjected to metagenomics analysis that revealed the presence of eleven viruses putatively belonging to families Partitiviridae, Hypoviridae, Megabirnaviridae, and orders Tymovirales and Bunyavirales, in addition to one ourmia-like virus plus other two unclassified virus species. Moreover, a sequence with 98% nucleotide identity to plant endornavirus Phaseolus vulgaris alphaendornavirus 1 has been identified in the Entoleuca sp. isolates. Concerning the virome composition, the four isolates only differed in the presence of the bunyavirus and the ourmia-like virus, while all other viruses showed common patterns. Specific primers allowed the detection by RT-PCR of these viruses in a collection of Entoleuca sp. and Rosellinia necatrix isolates obtained from roots of avocado trees. Results indicate that intra- and interspecies horizontal virus transmission occur frequently in this pathosystem. This research was funded by the Spanish Ministry of Science and Innovation project AGL2014-52518-C2-2-R and the IFAPA projects AVA201301.13 and AVA201601.14, co-financed by FEDER. |
| Databáze: | OpenAIRE |
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