Laboratory scale cultivation of Salinispora tropica in shake flasks and mechanically stirred bioreactors
| Autor: | Martin Halecky, Flavia Marinelli, Elisa Binda, Zuzana Jezkova, Tomas Potocar, Tomáš Brányik |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Nitrogen Biomass chemistry.chemical_element Bioengineering Flask and bioreactor cultivation complex mixtures 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Laboratory flask Bioreactors 010608 biotechnology Nitrogen sources Bioreactor Yeast extract Biomass growth kinetics Mechanical Phenomena biology Chemistry Salinispora tropica Temperature Micromonosporaceae General Medicine equipment and supplies biology.organism_classification Pulp and paper industry Culture Media Oxygen Glucose 030104 developmental biology Batch Cell Culture Techniques Seawater Biotechnology |
| Zdroj: | Biotechnology Letters. 43:1715-1722 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-021-03121-1 |
| Popis: | Marine actinomycetes from the genus Salinispora have an unexploited biotechnological potential. To accurately estimate their application potential however, data on their cultivation, including biomass growth kinetics, are needed but only incomplete information is currently available. This work provides some insight into the effect of temperature, salinity, nitrogen source, glucose concentration and oxygen supply on growth rate, biomass productivity and yield of Salinispora tropica CBN-440T. The experiments were carried out in unbaffled shake flasks and agitated laboratory-scale bioreactors. The results show that the optimum growth temperature lies within the range 28–30 °C, salinity is close to sea water and the initial glucose concentration is around 10 g/L. Among tested nitrogen sources, yeast extract and soy peptone proved to be the most suitable. The change from unbaffled to baffled flasks increased the volumetric oxygen transfer coefficient (kLa) as did the use of agitated bioreactors. The highest specific growth rate (0.0986 h−1) and biomass productivity (1.11 g/L/day) were obtained at kLa = 28.3 h−1. A further increase in kLa was achieved by increasing stirrer speed, but this led to a deterioration in kinetic parameters. Improvement of S. tropica biomass growth kinetics of was achieved mainly by identifying the most suitable nitrogen sources and optimizing kLa in baffled flasks and agitated bioreactors. |
| Databáze: | OpenAIRE |
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