Stabilization of Xist RNA mediates initiation of X chromosome inactivation
| Autor: | Gian-Carlo Alghisi, Sohaila Rastan, Neil Brockdorff, Sarah M. Duthie, Tatyana B. Nesterova, Emma J. Formstone, Alistair E. T. Newall, Steven A. Sheardown, Ruth M Arkell, Colette M. Johnston |
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| Rok vydání: | 1997 |
| Předmět: |
Male
RNA Untranslated Transcription Genetic Biology General Biochemistry Genetics and Molecular Biology X-inactivation Mice Transcription (biology) Dosage Compensation Genetic Animals RNA Messenger Skewed X-inactivation X chromosome Alleles Cells Cultured Nucleic Acid Synthesis Inhibitors Dosage compensation Models Genetic Biochemistry Genetics and Molecular Biology(all) Stem Cells RNA Cell Differentiation Molecular biology Blastocyst Gene Expression Regulation Dactinomycin XIST Female RNA Long Noncoding Tsix Transcription Factors |
| Zdroj: | Cell. 91(1) |
| ISSN: | 0092-8674 |
| Popis: | The onset of X inactivation is preceded by a marked increase in the level of Xist RNA. Here we demonstrate that increased stability of Xist RNA is the primary determinant of developmental up-regulation. Unstable transcript is produced by both alleles in XX ES cells and in XX embryos prior to the onset of random X inactivation. Following differentiation, transcription of unstable RNA from the active X chromosome allele continues for a period following stabilization and accumulation of transcript on the inactive X allele. We discuss the implications of these findings in terms of models for the initiation of random and imprinted X inactivation. |
| Databáze: | OpenAIRE |
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