Most Cleaved Anti-Müllerian Hormone Binds Its Receptor in Human Follicular Fluid but Little Is Competent in Serum
| Autor: | Paul Carmillo, Joëlle Taieb, Richard L. Cate, Chrystèle Racine, Alice Pierre, Rodolfo Rey, R. Blake Pepinsky, Nathalie di Clemente, Joëlle Cohen-Tannoudji, Renato Fanchin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Anti-Mullerian Hormone Male endocrine system medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD endocrine system diseases Receptors Peptide Endocrinology Diabetes and Metabolism Clinical Biochemistry testis Biochemistry 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine medicine AMH PCOS Humans Receptor Child Cleavage Body fluid 030219 obstetrics & reproductive medicine biology urogenital system Biochemistry (medical) Anti-Müllerian hormone purl.org/becyt/ford/3.1 [https] Bioquímica y Biología Molecular Polycystic ovary Follicular fluid female genital diseases and pregnancy complications Follicular Fluid Blot Medicina Básica 030104 developmental biology biology.protein purl.org/becyt/ford/3 [https] Female Antibody Receptors Transforming Growth Factor beta hormones hormone substitutes and hormone antagonists Hormone Polycystic Ovary Syndrome Protein Binding |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1945-7197 |
| Popis: | Context: Anti-Müllerian hormone (AMH) is an important clinical marker for diagnosing and assessing the reproductive status and/or disorders in men and women. Most studies have not distinguished between levels of inactive AMH precursor and the cleaved noncovalent complex that binds the AMH type II receptor (AMHRII) and initiates signaling. Objective: The objective of the study was to measure the levels of AMH cleavage and bioactivity in human body fluids. Design, Setting, and Patients: AMH cleavage levels and bioactivity were measured in the serum of six boys and in the follicular fluid and serum of nine control women and 13 women with the polycystic ovary syndrome (PCOS). Main Outcome Measures: AMH cleavage levels were measured by capturing AMH with an anti- AMH antibody, followed by Western blotting. The bioactivity of cleaved AMH was assessed with an ELISA that measures the levels of AMH capable of binding AMHRII. Results: PCOS women have an elevated level of AMH cleavage in their follicular fluid (24% vs 8% in controlwomen),andmostofthecleavedAMHcanbindAMHRII.Higherlevels of cleavage areobserved in female (60%) and male (79%) serum, but very little of the cleaved AMH can bind AMHRII. Conclusions: These results support an autocrine role for AMH in the pathophysiology of PCOS in the follicle. In addition,theyindicate thatAMHundergoesinteractions or structuralchangesaftercleavage that prevent receptor binding, meaning, unexpectedly, that the level of cleaved AMH in biological fluids does not always reflect the level of bioactive AMH. Fil: Pierre, Alice. Inserm; Francia. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia Fil: Racine, Chrystèle. Inserm; Francia. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia Fil: Rey, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina Fil: Fanchin, Renato. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia Fil: Taieb, Joëlle. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia Fil: Cohen Tannoudji, Joëlle. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia Fil: Carmillo, Paul. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Pepinsky, R. Blake. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Cate, Richard L.. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia Fil: Di Clemente, Nathalie. Inserm; Francia. Université Paris Diderot - Paris 7; Francia. Centre National de la Recherche Scientifique; Francia |
| Databáze: | OpenAIRE |
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