Development and in-house validation of a rapid and simple to use ELISA for the detection and measurement of the mycotoxin sterigmatocystin

Autor: Katrina Campbell, Christopher T. Elliott, Nermin Sajic, Willem Haasnoot, Michalina Oplatowska-Stachowiak, Martin Salden, Claudine Reiring, Catherine Brabet
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Agricultural commodity
Aflatoxin
Time Factors
genetic structures
Sterigmatocystin
01 natural sciences
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Mice
Limit of Detection
Screening method
Food science
Triticum
2. Zero hunger
Immunoassay
Mycotoxin
medicine.diagnostic_test
Antibodies
Monoclonal
food and beverages
04 agricultural and veterinary sciences
040401 food science
3. Good health
Q03 - Contamination et toxicologie alimentaires
Hazard Analysis and Critical Control Points
Biology
Quechers
Zea mays
Food safety
0404 agricultural biotechnology
SDG 3 - Good Health and Well-being
Enzyme-linked immunosorbent assay
BU Authenticity & Bioassays
medicine
Animals
VLAG
business.industry
010401 analytical chemistry
Oryza
0104 chemical sciences
BU Authenticiteit & Bioassays
chemistry
U30 - Méthodes de recherche
business
Zdroj: Analytical and Bioanalytical Chemistry, 410(12), 3017-3023
Oplatowska-Stachowiak, M, Reiring, C, Sajic, N, Haasnoot, W, Brabet, C, Campbell, K, Elliott, C T & Salden, M 2018, ' Development and in-house validation of a rapid and simple to use ELISA for the detection and measurement of the mycotoxin sterigmatocystin ', Analytical and Bioanalytical Chemistry . https://doi.org/10.1007/s00216-018-0988-8
Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry 410 (2018) 12
ISSN: 1618-2642
DOI: 10.1007/s00216-018-0988-8
Popis: Sterigmatocystin (STG) is a highly toxic secondary fungal metabolite structurally closely related to the well-known carcinogenic aflatoxins. Its presence has been reported in grains and grain-based products as well as in other foodstuffs like nuts, green coffee beans, spices, beer and cheese. Due to the lack of suitable data on the occurrence of STG, in 2013, the European Food Safety Authority (EFSA) could not characterise its risk for human health and recommended that more data on STG in food and feed needed to be collected. In order to provide a new tool for the specific detection of STG, a competitive enzyme-linked immunosorbent assay (ELISA) was developed, optimised and validated in this study based on a sensitive monoclonal antibody specific to STG with no cross-reactivity with aflatoxins. The sample preparation method for rice, wheat and maize was based on a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach. The assay was validated for the detection of STG in rice, wheat and maize in accordance with the guidelines for validation of semi-quantitative screening methods included in Commission Regulation (EU) 519/2014. The screening target concentration (STC) was set at 1.5 μg/kg. The cutoffs for rice, wheat and maize were 1.2, 1.2 and 1.3 μg/kg and the false suspected rates were 0.34, 1.15 and 0.78%, respectively. Good correlation was found between the results obtained by the STG ELISA and LC-MS/MS method for naturally contaminated rice samples. This validated method can be applied as a sensitive and high-throughput screening for the presence of STG in a range of agricultural commodities. Graphical abstract A new enzyme-linked immunosorbent assay based on an antibody specific to sterigmatocystin for the detection of this mycotoxin in corn, wheat and rice.
Databáze: OpenAIRE
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