Histochemical evaluation of postnatal lectin-binding sites in the mouse prostate
| Autor: | Kazuya Yoshinaga, Kentaro Sakuda, Ryoki Muragishi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Prostatic Diseases medicine.medical_specialty Cell type Cellular differentiation Cell Mice 03 medical and health sciences Prostate Lectins Internal medicine medicine Animals Soybean agglutinin Binding Sites biology Lectin Immunohistochemistry Molecular biology Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure biology.protein Anatomy |
| Zdroj: | Okajimas Folia Anatomica Japonica. 92:61-66 |
| ISSN: | 1881-1736 0030-154X |
| DOI: | 10.2535/ofaj.92.61 |
| Popis: | The prostate is a male accessory genital gland that plays an essential role in reproductive function. To understand the cytological characteristics of differentiating prostatic cells, we used lectin histochemistry combined with immunohistochemistry to examine the distribution of lectin-binding sites on prostatic cells during postnatal development in the mouse. During postnatal development, Hippeastrum Hybrid Lectin (HHL) lectin reacted consistently with the luminal cells of all prostatic lobes (regions), whereas the Ricinus Communis Agglutinin I (RCA-I) and Soybean Agglutinin (SBA) lectins showed remarkable differences with age, region, and cell type. We found that the lectin-binding pattern in differentiating prostatic cells acquired adult characteristics around 3 weeks after birth. The results indicate that prostatic cell differentiation during postnatal development in mice is characterized by the presence of cell- and region-specific lectin-binding sites in the prostate, suggesting that there may also be cellular and regional differences in their function. Furthermore, some lectins (HHL, RCA-I, and SBA) could provide useful markers for research into cell differentiation and for the pathological evaluation of prostatic diseases or in the diagnosis of male infertility. |
| Databáze: | OpenAIRE |
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