DNA microarray analysis of white adipose tissue from obese (fa/fa) Zucker rats treated with a β3-adrenoceptor agonist, KTO-7924
| Autor: | Masayuki Isaji, Akane Matsuzawa, Masuo Akahane, Toshiki Homma, Fumiki Oana, Hiroo Takeda, Satoshi Akahane |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Adrenergic beta-3 Receptor Agonists White adipose tissue Biology Insulin resistance Adipose Tissue Brown Lipid oxidation Internal medicine Brown adipose tissue medicine Animals Obesity Oligonucleotide Array Sequence Analysis Pharmacology chemistry.chemical_classification Gene Expression Profiling Insulin nutritional and metabolic diseases Fatty acid Lipid metabolism Lipid Metabolism medicine.disease Adrenergic Agonists Thermogenin Rats Rats Zucker medicine.anatomical_structure Endocrinology Adipose Tissue chemistry lipids (amino acids peptides and proteins) Insulin Resistance hormones hormone substitutes and hormone antagonists |
| Zdroj: | Pharmacological Research. 52:395-400 |
| ISSN: | 1043-6618 |
| DOI: | 10.1016/j.phrs.2005.05.014 |
| Popis: | We aimed to examine the effects of KTO-7924 (β3-adrenoceptor agonist) on lipid metabolism and mRNA expressions in retroperitoneal white adipose tissue (RP WAT) in obese (fa/fa) Zucker rats using DNA microarray. Oral KTO-7924 for 28 days significantly decreased RP WAT weight, plasma triglyceride, free fatty acid, and insulin, and improved insulin resistance in oral glucose tolerance tests. In RP WAT of KTO-7924-treated rats, DNA microarray analysis revealed specifically enhanced mRNA expressions of uncoupling protein 1 (UCP1) and cytochrome c oxidase subunit VIII-H (COX8H), which are reportedly highly expressed in brown adipose tissue (BAT). Since these mRNA expression levels in RP WAT were significantly lower in obese (fa/fa) Zucker rats than in lean Zucker rats, these genes may be important in lipid metabolism. Our results imply that in obese (fa/fa) Zucker rats, continuous stimulation of β3-adrenoceptors by KTO-7924 causes BAT-like adipocytes to appear in RP WAT, and improves lipid metabolism. |
| Databáze: | OpenAIRE |
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