Cellular interactions between social experience, alcohol sensitivity, and GABAergic inhibition in a crayfish neural circuit
| Autor: | Norma L Pena-Flores, Jens Herberholz, Lucy Venuti |
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| Rok vydání: | 2021 |
| Předmět: |
Agonist
medicine.medical_specialty Pyridines Physiology medicine.drug_class Astacoidea 01 natural sciences 010104 statistics & probability 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Interneurons Postsynaptic potential Internal medicine medicine Animals GABA-A Receptor Antagonists GABAergic Neurons 0101 mathematics Social Behavior GABA Agonists Neuropharmacology Ethanol musculoskeletal neural and ocular physiology General Neuroscience Antagonist Isoxazoles Synaptic Potentials Crayfish Phosphinic Acids Endocrinology nervous system chemistry Muscimol GABAergic 030217 neurology & neurosurgery Research Article Picrotoxin |
| Zdroj: | J Neurophysiol |
| ISSN: | 1522-1598 0022-3077 |
| DOI: | 10.1152/jn.00519.2020 |
| Popis: | We report here that prior social experience modified the behavioral responses of adult crayfish to acute alcohol exposure. Animals housed individually for 1 wk before alcohol exposure were less sensitive to the intoxicating effects of alcohol than animals housed in groups, and these differences are based on changes in the nervous system rather than differences in alcohol uptake. To elucidate the underlying neural mechanisms, we investigated the neurophysiological responses of the lateral giant (LG) interneurons after alcohol exposure. Specifically, we measured the interactions between alcohol and different GABA(A)-receptor antagonists and agonists in reduced crayfish preparations devoid of brain-derived tonic GABAergic inhibition. We found that alcohol significantly increased the postsynaptic potential of the LG neurons, but contrary to our behavioral observations, the results were similar for isolated and communal animals. The GABA(A)-receptor antagonist picrotoxin, however, facilitated LG postsynaptic potentials more strongly in communal crayfish, which altered the neurocellular interactions with alcohol, whereas TPMPA [(1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid], an antagonist directed against GABA(A)-receptors with ρ subunits, did not produce any effects. Muscimol, an agonist for GABA(A)-receptors, blocked the stimulating effects of alcohol, but this was independent of prior social history. THIP [4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol], an agonist directed against GABA(A)-receptors with δ subunits, which were not previously known to exist in the LG circuit, replicated the suppressing effects of muscimol. Together, our findings provide strong evidence that alcohol interacts with the crayfish GABAergic system, and the interplay between prior social experience and acute alcohol intoxication might be linked to changes in the expression and function of specific GABA(A)-receptor subtypes. NEW & NOTEWORTHY The complex interactions between alcohol and prior social experience are still poorly understood. Our work demonstrates that socially isolated crayfish exhibit lower neurobehavioral sensitivity to acute ethanol compared with communally housed animals, and this socially mediated effect is based on changes in the nervous systems rather than on differences in uptake or metabolism. By combining intracellular neurophysiology and neuropharmacology, we investigated the role of the main inhibitory neurotransmitter GABA, and its receptor subtypes, in shaping this process. |
| Databáze: | OpenAIRE |
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