Insulin sensitivity of adipocytes from inbred mouse strains resistant or sensitive to diet-induced obesity
| Autor: | G. P. Eberhart, R. L. Atkinson, D. B. West, C. N. Boozer |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Physiology medicine.medical_treatment Adipose tissue Mice Inbred Strains Carbohydrate metabolism Biology Deoxyglucose chemistry.chemical_compound Mice Mice Inbred AKR Inbred strain Species Specificity Physiology (medical) Internal medicine Adipocyte medicine Adipocytes Animals Insulin Obesity Pancreatic hormone Cells Cultured Epididymis Analysis of Variance Body Weight Glucose transporter Organ Size medicine.disease Dietary Fats Kinetics Endocrinology Glucose chemistry |
| Zdroj: | The American journal of physiology. 266(5 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | We evaluated insulin sensitivity in epididymal adipocytes from two mouse strains shown to be either sensitive (AKR/J, n = 14) or resistant (SWR/J, n = 12) to the development of obesity when fed a high-fat diet. Half of each strain was fed a chow (CH) diet (12% fat), and half received a sweetened condensed milk (CM) diet (33% fat). After 1 wk, epididymal adipose depots were removed and digested with collagenase, and glucose transport was measured with labeled 2-deoxyglucose. Plasma glucose and insulin were slightly higher in AKR/J than SWR/J mice (glucose: 139.7 vs. 118.8 mg/dl, P < 0.06; insulin: 3.45 vs. 2.99 ng/ml, P < 0.04). One week of high-fat feeding increased adipose depot mass and carcass lipid in both strains to approximately the same extent. Adipocytes from AKR/J mice had greater insulin-stimulated glucose transport compared with SWR/J mice at both submaximal and maximal insulin levels (P < 0.0001). Short-term feeding of the high-fat diet increased AKR/J adipocyte insulin sensitivity but decreased the sensitivity of SWR/J adipocytes to insulin. The differences in adipocyte insulin sensitivity between strains were not explained by differences in adipocyte cell size. Access to the high-fat CM diet for 12 wk increased total dissected adipose depot size by 209% in the AKR/J mice and 82% in the SWR/J mice. These data clearly demonstrate that the two strains differ in adipocyte insulin sensitivity as well as sensitivity to dietary obesity. Increased adipocyte insulin sensitivity could contribute to a predisposition to increase adipose tissue lipid stores with diets high in fat content. |
| Databáze: | OpenAIRE |
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