Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil

Autor: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Eric Fabrício Marialva, Antonio Marques Pereira Júnior, Ana Paula de Azevedo dos Santos, Moreno S. Rodrigues, Carolina Bioni Garcia Teles
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Entomology
Skin Leishmaniasis
Physiology
Fauna
Phlebotominae
HSP72 Heat-Shock Proteins
Polymerase Chain Reaction
law.invention
Abundance (ecology)
law
Disease Carrier
Kinetoplastida
Polymerase chain reaction
Leishmania
Diversity
Trypanosomatidae
Ecology
Cutaneous Leishmaniasis
Richness
Hexapoda
Vectors
Nyssomyia
Psychodopygus Davisi
Infectious Diseases
Female
Tracheitis
Psychodopygus
Brazil
Polymorphism
Restriction Fragment Length
Human
Dna Sequence
Gene Sequence
Trichophoromyia Ubiquitalis
Kinetoplast
Morphotype
Heat Shock Protein 72
Protozoal Dna
Species Difference
Biology
Ecological Phenomena And Functions
Hsp72 Heat-shock Proteins
Disease Transmission
Species Specificity
Dna Extraction
parasitic diseases
medicine
Genetics
Animals
Psychodidae
Ecosystem
Animal
Research
Amazon environments
Brasil
Nyssomyia Antunesi
Diptera
fungi
Leishmaniasis
Dna
DNA
Protozoan
medicine.disease
biology.organism_classification
Nonhuman
Insect Vectors
Dna
Protozoan
Metabolism
Gene Expression Regulation
Parasitology
Species richness
Species Richness
Zdroj: Repositório Institucional do INPA
Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
Parasites & Vectors
Popis: Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality. Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA. © 2015 Pereira et al.; licensee BioMed Central.
Databáze: OpenAIRE
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