[Brain-heart media for blood cultures.]
| Autor: | V I Kocherovets, O Y Borisova, A Y Mironov, N M Kargaltseva |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Microorganism Russia 03 medical and health sciences 0302 clinical medicine Fish meal food Bloodstream infection medicine Agar Humans Microaerophile Blood culture Food science 0303 health sciences medicine.diagnostic_test Bacteria 030306 microbiology Chemistry Biochemistry (medical) Brain Heart General Medicine Culture Media Medical Laboratory Technology Blood Culture Anaerobic exercise 030215 immunology |
| Zdroj: | Klinicheskaia laboratornaia diagnostika. 65(6) |
| ISSN: | 0869-2084 |
| Popis: | When diagnosing bloodstream infection (BI) the culture medium is the basis for growth of microorganisms and obtaining the blood culture. Pancreatic digest from fish meal is the basis of all culture media in Russia. In European countries brain-heart media (BHM) are used for detecting microorganisms in blood. In Russia BHM is not produced. The aim is to work out the formulation and the way of the BHM (broth and agar) preparation in order to improve the efficiency of obtaining blood culture. There were defined the physical and chemical indices and biological parameters of the BHM. The microbiological control of the BHM was carried out by diagnostic study of cardiological patients’ blood. On the basis of the developed technique of the brain-heart extraction (BHE) preparation there was created the liquid and agar BHM (LBHM, BHA). The LBHM was poured into bottles which then were filled with the inert gas. The bottles were closed with rubber stoppers and rolled in metal caps became a closed system. Microbiological qualities of LBHM were tested on 260 blood samples and thioglycollate medium (TGM) and LBHM. Aerobic microorganisms grew in LBHM 2,4 times more often than in TGM. The microaerophilic microbes grew in LBHM 3,2 times more often than in TGM. Anaerobic microbes did not show any growth in TGM, (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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