Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)
| Autor: | Diego Luis Ribeiro, José Rueff, Bruno Gomes, Gustavo Rafael Mazzaron Barcelos, Adriana Ladeira de Araújo, Lusânia Maria Greggi Antunes, Fernando Barbosa, Marilia Ladeira de Araújo, Bruno Lemos Batista, Nathalia de Assis Aguilar Duarte, António Sebastião Rodrigues, Paula Pícoli Devóz |
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| Přispěvatelé: | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Centre for Toxicogenomics and Human Health (ToxOmics) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
lead
lcsh:QH426-470 epigenetics Global DNA Methylation Profile toxicity occupational exposure Biology Non-coding RNA Molecular biology ncRNA Chromatin remodeling TOXICOLOGIA chemistry.chemical_compound lcsh:Genetics chemistry microRNA DNA methylation TaqMan Genetics Molecular Medicine Epigenetics Genetics (clinical) DNA Original Research miRNA |
| Zdroj: | Frontiers in Genetics Frontiers in Genetics, Vol 12 (2021) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | 2018/24643-1 (São Paulo Research Foundation, FAPESP, Brazil), by the National Council for Scientific and Technological Development (Brazil) and by grant UID-BIM-00009-2020 (Foundation for Science and Technology, FCT, Portugal). Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb. publishersversion published |
| Databáze: | OpenAIRE |
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