Comparative analysis of paracrine immunotherapy in experimental brain tumors
| Autor: | Betty Tyler, Henry Brem, Maciej S. Lesniak, Prakash Sampath, Francesco DiMeco, Michael P. Viglione, Drew M. Pardoll |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Brain tumor Biology CD8-Positive T-Lymphocytes Transfection Mice Cell Line Tumor Paracrine Communication medicine Cytotoxic T cell Animals Melanoma Brain Neoplasms Interleukin Granulocyte-Macrophage Colony-Stimulating Factor General Medicine Immunotherapy Genetic Therapy medicine.disease Eosinophils Mice Inbred C57BL Survival Rate Cytokine Interleukin-2 Surgery Neurology (clinical) Interleukin-4 Infiltration (medical) CD8 Cell Division Neoplasm Transplantation |
| Zdroj: | Neurosurgical focus. 9(6) |
| ISSN: | 1092-0684 |
| Popis: | Object Local delivery of cytokines has been shown to have a potent antitumor activity against a wide range of malignant brain tumors. In this study, the authors examined the efficacy of treating central nervous system (CNS) tumors by transfecting poorly immunogenic B16/F10 melanoma cells with interleukin (IL)-2, IL-4, or granulocyte-macrophage–colony stimulating factor (GM-CSF) gene, and using these cells to deliver the cytokine locally at the site of the CNS tumor. The object was to determine which cytokine would possess the greatest antitumor activity and to further elucidate its mechanism of action. Methods The transfected B16/F10 cells were irradiated to prevent replication and injected intracranially into C57BL/6 mice (10 mice per group) along with nonirradiated, nontransfected B16/F10 (wild-type) melanoma cells. Sixty percent of mice treated with IL-2 (p < 0.001 compared with control) and 10% treated with IL-4 (median survival = 31 days, p < 0.001 compared with control) were long term survivors (> 120 days). The median survival for animals treated with GM-CSF was 22 days with no long term survivors (p = 0.01 compared with control). Control animals that received only wild-type cells had a median survival of 18 days (range 15–20 days). Histopathological examination of brains from animals killed at different times showed minimal infiltration of tumor cells in the IL-2 group, moderate infiltration of tumor cells in the IL-4 group, and gross tumor invasion and tissue necrosis in the GM-CSF group. Animals treated with IL-2 showed a strong CD8 T cell–mediated response, whereas IL-4 evoked a prominent eosinophilic infiltrate in the area of the tumor. Conclusions High levels of locally expressed IL-2 rather than IL-4 or GM-CSF stimulate a strong immunological cytotoxic antitumor response that leads to significant prolongation of survival in mice challenged with B16/F10 intracranial melanoma tumor cells. Consequently, IL-2 may be a superior candidate for use in paracrine immunotherapy. |
| Databáze: | OpenAIRE |
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