Transplantation of embryonic serotonin immunoreactive neurons into the transected spinal cord of adult monkey (Macaca fascicularis)
| Autor: | F. Sandillon, F. Famose, P. Poulat, Alain Privat, Marie-Jeanne Drian, N. Rajaofetra, Michel Geffard, B. Verschuere, Norbert König, L. Marlier, D. Gouy |
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| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty Serotonin Central nervous system Cyclosporins Biology Grey matter Serotonergic White matter Fetal Tissue Transplantation medicine Animals Brain Tissue Transplantation Molecular Biology Neurons General Neuroscience Graft Survival Embryo Anatomy Spinal cord Transplantation Rhombencephalon Macaca fascicularis medicine.anatomical_structure Spinal Cord Neurology (clinical) Developmental Biology |
| Zdroj: | Brain research. 572(1-2) |
| ISSN: | 0006-8993 |
| Popis: | Five adult monkeys(Macaca fascicularis) underwent a total section of the spinal cord at the thoracic level (T6). Four of them received a daily treatment with cyclosporin (10 mg/kg). Ten days later, two animals treated with cyclosporin and one wiithout cyclosporin received at T8 and T10 levels an injection of a cell suspension prepared from the rhombencephalon of a 40-day-old macaque embryo. Two control animals received one injection of Hank's balanced salt solution. The animals were sacrificed after 2 months (one grafted and one control) and 3 months (two grafted and one control), and their spinal cord was processed for the immunocytochemical detection of serotonin using light and electron microscopy. After 2 months of survival, serotonergic neurons had survived and developed within the transplant. Three months after transplantation, in the animal treated with cyclosporin, serotonergic neurons were found to survive with their axons growing into the host grey matter and establishing axosomatic and axodendritic synapses in the ventral horn. If the graft was isolated in the white matter no fibers were seen invading the grey matter. |
| Databáze: | OpenAIRE |
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