Protein carbonylation during natural leaf senescence in winter wheat, as probed by fluorescein-5-thiosemicarbazide
| Autor: | Matthieu Bagard, M. Havé, Anne Repellin, L. Leitao, Jean-François Castell |
|---|---|
| Přispěvatelé: | Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Senescence Nitrogen [SDV]Life Sciences [q-bio] Proteolysis Protein Carbonylation natural senescence Plant Science Oxidative phosphorylation Protein degradation Biology Protein oxidation Sensitivity and Specificity 01 natural sciences 03 medical and health sciences chemistry.chemical_compound wheat medicine protein oxidation Fluorescein Cellular Senescence Triticum Ecology Evolution Behavior and Systematics 030304 developmental biology 2. Zero hunger 0303 health sciences Staining and Labeling medicine.diagnostic_test fungi food and beverages General Medicine Fluoresceins Phenylhydrazines Plant Leaves fluorescein-5-thiosemicarbazide Biochemistry chemistry proteolytic activity Edible Grain Carbonylation 010606 plant biology & botany |
| Zdroj: | Plant Biology Plant Biology, Wiley, 2015, 17, pp.973-979. ⟨10.1111/plb.12315⟩ |
| ISSN: | 1435-8603 1438-8677 |
| DOI: | 10.1111/plb.12315 |
| Popis: | Leaf senescence is characterised by a massive degradation of proteins in order to recycle nitrogen to other parts of the plant, such as younger leaves or developing grain/seed. Protein degradation during leaf senescence is a highly regulated process and it is suggested that proteins to be degraded are marked by an oxidative modification (carbonylation) that makes them more susceptible to proteolysis. However, there is as yet no evidence of an increase in protein carbonylation level during natural leaf senescence. The aim of our study was thus to monitor protein carbonylation level during the process of natural senescence in the flag leaf of field-grown winter wheat plants. For this purpose, we adapted a fluorescence-based method using fluorescein-5-thiosemicarbazide (FTC) as a probe for detecting protein carbonyl derivatives. As used for the first time on plant material, this method allowed the detection of both quantitative and qualitative modifications in protein carbonyl levels during the last stages of wheat flag leaf development. The method described herein represents a convenient, sensitive and reproducible alternative to the commonly used 2,4-dinitrophenylhydrazine (DNPH)-based method. In addition, our analysis revealed changes in protein carbonylation level during leaf development that were associated with qualitative changes in protein abundance and carbonylation profiles. In the senescing flag leaf, protein carbonylation increased concomitantly with a stimulation of endoproteolytic activity and a decrease in protein content, which supports the suggested relationship between protein oxidation and proteolysis during natural leaf senescence |
| Databáze: | OpenAIRE |
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