Anomalous incisor morphology indicates tissue-specific roles for Tfap2a and Tfap2b in tooth development
| Autor: | Emily D. Woodruff, Martin J. Cohn, Galaxy C. Gutierrez, Trevor Williams, Eric Van Otterloo |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Molar Mesenchyme Biology Article Epithelium TFAP2A Animals Genetically Modified Mesoderm Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system Incisor medicine Animals Craniofacial Molecular Biology Alleles 030304 developmental biology 0303 health sciences Dentition Tooth Germ Cell Biology Dental lamina Cell biology stomatognathic diseases medicine.anatomical_structure Transcription Factor AP-2 Odontogenesis Cusp (anatomy) Female Gene Deletion 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
| Zdroj: | Dev Biol |
| ISSN: | 0012-1606 |
| Popis: | Mice possess two types of teeth that differ in their cusp patterns; incisors have one cusp and molars have multiple cusps. The patterning of these two types of teeth relies on fine-tuning of the reciprocal molecular signaling between dental epithelial and mesenchymal tissues during embryonic development. The AP-2 transcription factors, particularly Tfap2a and Tfap2b, are essential components of such epithelial-mesenchymal signaling interactions that coordinate craniofacial development in mice and other vertebrates, but little is known about their roles in the regulation of tooth development and shape. Here we demonstrate that incisors and molars differ in their temporal and spatial expression of Tfap2a and Tfap2b. At the bud stage, Tfap2a is expressed in both the epithelium and mesenchyme of the incisors and molars, but Tfap2b expression is restricted to the molar mesenchyme, only later appearing in the incisor epithelium. Tissue-specific deletions show that loss of the epithelial domain of Tfap2a and Tfap2b affects the number and spatial arrangement of the incisors, notably resulting in duplicated lower incisors. In contrast, deletion of these two genes in the mesenchymal domain has little effect on tooth development. Collectively these results implicate epithelial expression of Tfap2a and Tfap2b in regulating the extent of the dental lamina associated with patterning the incisors and suggest that these genes contribute to morphological differences between anterior (incisor) and posterior (molar) teeth within the mammalian dentition. |
| Databáze: | OpenAIRE |
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