Absence of Yps7p, a putative glycosylphosphatidylinositol-linked aspartyl protease inPichia pastoris, results in aberrant cell wall composition and increased osmotic stress resistance
| Autor: | Shuai Su, Duan Zuoying, Jian Jin, Gong Xiaohai, Bo Guan, Fengxiang Chen, Yun Chen, Huazhong Li, Jianyong Lei |
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| Rok vydání: | 2012 |
| Předmět: |
Osmosis
Aspartic Acid Proteases beta-Glucans Osmotic shock Glycosylphosphatidylinositols Mutant Saccharomyces cerevisiae Chitin Calcofluor-white Applied Microbiology and Biotechnology Microbiology Pichia Pichia pastoris Fungal Proteins Cell wall Microscopy Electron Transmission Cell Wall Stress Physiological biology Computational Biology General Medicine biology.organism_classification Molecular biology Phenotype Microscopy Fluorescence Mutation Proteoglycans Intracellular Plasmids |
| Zdroj: | FEMS Yeast Research. 12:969-979 |
| ISSN: | 1567-1356 |
| DOI: | 10.1111/1567-1364.12002 |
| Popis: | Recently, studies performed on Saccharomyces cerevisiae and Candida albicans have confirmed the importance of fungal glycosylphosphatidylinositol (GPI)-anchored aspartyl proteases (yapsins) for cell-wall integrity. Genome sequence annotation of Pichia pastoris also revealed seven putative GPI-anchored aspartyl protease genes. The five yapsin genes assigned as YPS1, YPS2, YPS3, YPS7 and MKC7 in P. pastoris were disrupted. Among these putative GPI-linked aspartyl proteases, disruption of PpYPS7 gene confers the Ppyps7Δ mutant cell increased resistance to cell wall perturbing reagents congo red, calcofluor white (CW) and sodium dodecyl sulfate. Quantitative analysis of cell wall components shows lower content of chitin and increased amounts of β-1,3-glucan. Further staining of the cell with CW demonstrates that disruption of PpYPS7 gene causes a reduction of the chitin content in lateral cell wall. Consistently, transmission electron micrographs show that the inner layer of mutant cell wall, mainly composed of chitin and β-1, 3-glucan, is much thicker than that in parental strain GS115. Additionally, Ppyps7Δ mutant also exhibits increased osmotic resistance compared with parental strain GS115. This could be due to the dramatically elevated intracellular glycerol level in Ppyps7Δ mutant. These results suggest that PpYPS7 is involved in cell wall integrity and response to osmotic stress. |
| Databáze: | OpenAIRE |
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