Effects of aminopeptidase P inhibition on kinin-mediated vasodepressor responses
| Autor: | Luis A. Carbini, Shin-Ichi Kitamura, A. Guillermo Scicli, William H. Simmons |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Physiology Bradykinin Neuropeptide Vasodilation Angiotensin-Converting Enzyme Inhibitors Blood Pressure Peptidyl-Dipeptidase A Aminopeptidases Aortic Coarctation chemistry.chemical_compound Lisinopril Physiology (medical) Internal medicine medicine Animals Protease Inhibitors Rats Wistar Aorta chemistry.chemical_classification biology business.industry Angiotensin-converting enzyme Metabolism Kinin Acetylcholine Rats Endocrinology Enzyme chemistry Injections Intra-Arterial Vasoconstriction Hypertension Injections Intravenous biology.protein Angiotensin I Cardiology and Cardiovascular Medicine business Peptides medicine.drug |
| Zdroj: | The American journal of physiology. 276(5) |
| ISSN: | 0002-9513 |
| Popis: | We studied in anesthetized rats whether aminopeptidase P (AMP) may be involved in bradykinin (BK) metabolism and responses. For this we inhibited AMP with the specific inhibitor apstatin (Aps). Studies were done with Aps alone or together with the angiotensin-converting enzyme inhibitor lisinopril (Lis). Aps increased the vasodepressor response to an intravenous bolus of BK (400 ng/kg): vehicle, −3.0 ± 0.7 mmHg; Aps, −7.8 ± 0.7 mmHg ( P < 0.01 vs. vehicle); Lis, −23.8 ± 1.8 mmHg; Aps + Lis, −37.5 ± 1.9 mmHg ( P < 0.01 vs. Lis). Aps did not affect the vasodepressor response to BK given into the descending aorta. Plasma BK increased only in Aps + Lis-treated rats (in pg/ml): control, 48.0 ± 1.4; Lis, 57.5 ± 7.6; Aps + Lis, 121.8 ± 30.6 ( P < 0.05 vs. control or Lis), whereas in rats infused with BK (400 ng ⋅ kg−1 ⋅ min−1for 5 min), Aps increased plasma BK (in pg/ml): control, 51.9 ± 2.5; Aps, 83.5 ± 20.5; Lis, 725 ± 225; Aps + Lis, 1,668 ± 318 ( P < 0.05, Aps vs. control and Lis vs. Aps + Lis). In rats with aortic coarctation hypertension, the acute antihypertensive effects of Aps plus Lis were greater than Lis alone ( P < 0.01). Hoe-140, a BK B2-receptor antagonist, abolished the difference. We concluded that in the rat AMP contributes to regulation of BK metabolism and responses. |
| Databáze: | OpenAIRE |
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