Engineered pH-Responsive Mesoporous Carbon Nanoparticles for Drug Delivery
| Autor: | Konstantinos Spyrou, Katharina Schmidt-Bleek, Miguel Gisbert-Garzarán, Dimitra Giasafaki, Theodore Steriotis, Georg N. Duda, María Vallet-Regí, Daniel Lozano, Georgia Charalambopoulou, Julia C. Berkmann, Miguel Manzano |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Cell Survival Polymers Nanoparticle Biocompatible Materials 02 engineering and technology 010402 general chemistry 01 natural sciences Article Ruthenium Mice In vivo Cell Line Tumor Animals Humans General Materials Science Cytotoxicity chemistry.chemical_classification Drug Carriers Materiales Polymer Mesoporous silica Carbocyanines Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Silicon Dioxide Controlled release Química inorgánica Carbon 0104 chemical sciences Mice Inbred C57BL Drug Liberation chemistry Drug delivery Biophysics Nanoparticles Nanocarriers 0210 nano-technology Porosity |
| Zdroj: | ACS Appl Mater Interfaces E-Prints Complutense. Archivo Institucional de la UCM instname E-Prints Complutense: Archivo Institucional de la UCM Universidad Complutense de Madrid ACS Applied Materials & Interfaces |
| Popis: | In this work, two types of mesoporous carbon particles with different morphology, size and pore structure have been functionalized with a self-immolative polymer sensitive to changes in pH and tested as drug nanocarriers. It is shown that their textural properties allow significantly higher loading capacity compared to typical mesoporous silica nanoparticles. In vial release experiments of a model Ru dye at pH 7.4 and 5 confirm the pH-responsiveness of the hybrid systems, showing that only small amounts of the cargo are released at physiological pH, whereas at slightly acidic pH (e.g. that of lysosomes) self-immolation takes place and a significant amount of the cargo is released. Cytotoxicity studies using human osteosarcoma cells show that the hybrid nanocarriers are not cytotoxic by themselves but induce significant cell growth inhibition when loaded with a chemotherapeutic drug such as doxorubicin. In preparation of an in vivo application, in vial responsiveness of the hybrid system to short-term pH-triggering is confirmed. The consecutive in vivo study shows no substantial cargo release over a period of 96 hours under physiological pH conditions. Short-term exposure to acidic pH releases an experimental fluorescent cargo during and continuously after the triggering period over 72 hours. |
| Databáze: | OpenAIRE |
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