Comparison of 'Polarization Inversion with Spin Exchange at Magic Angle' and 'Geometric Analysis of Labeled Alanines' Methods for Transmembrane Helix Alignment
| Autor: | Stanley J. Opella, Christopher V. Grant, Anna E. Daily, Roger E. Koeppe, Vitaly V. Vostrikov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Magic angle
Alanine Geometric analysis Ethanol Chemistry Bilayer Lipid Bilayers General Chemistry Polarization (waves) Biochemistry Molecular physics Amides Catalysis Article Protein Structure Secondary Transmembrane domain Colloid and Surface Chemistry Nuclear magnetic resonance Phosphatidylcholines Lipid bilayer Peptides Nuclear Magnetic Resonance Biomolecular |
| Popis: | Using the model alpha-helical peptide acetyl-GGALW5LALALALALALALW19LAGA-ethanolamide ("GWALP23"), we have compared the polarization inversion with spin exchange at magic angle method and geometric analysis of labeled alanines method for estimating the transmembrane helix orientation. For GWALP23 in bilayers of a short lipid, dilauroylphosphatidylcholine, we find general agreement between the two methods, with a static helix tilt of about 11degrees-13degrees with respect to the bilayer normal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|