Morphological and initial molecular characterization of Clogmia albipunctatus larvae (Diptera: Psychodidae) causing urinary myiasis in Egypt
| Autor: | Enas Abdelhameed Mahmoud Huseein, Haiam Mohammed Mahmoud Farrag, Ragaa A. Othman, Amal M. Almatary |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Life Cycles Ectoparasitic Infections RC955-962 Respiratory System medicine.disease_cause DNA barcoding Geographical Locations Database and Informatics Methods 0302 clinical medicine Larvae Arctic medicine. Tropical medicine RNA Ribosomal 16S Medicine and Health Sciences Cluster Analysis Animal Anatomy Child Phylogeny Data Management Larva Microscopy biology Cytochrome b Eukaryota Phylogenetic Analysis Cytochromes b Phylogenetics Insects Infectious Diseases Moths and Butterflies Child Preschool Egypt Female Public aspects of medicine RA1-1270 Anatomy Sequence Analysis Research Article Neglected Tropical Diseases Urologic Diseases medicine.medical_specialty Mitochondrial DNA Computer and Information Sciences Multiple Alignment Calculation Asia Adolescent Arthropoda Bioinformatics 030231 tropical medicine Zoology Spiracles Research and Analysis Methods DNA Mitochondrial DNA Ribosomal 03 medical and health sciences Myiasis Young Adult Molecular genetics Infestation Computational Techniques medicine Parasitic Diseases Animals DNA Barcoding Taxonomic Humans Evolutionary Systematics Psychodidae Taxonomy Evolutionary Biology fungi Public Health Environmental and Occupational Health Organisms Biology and Life Sciences NADH Dehydrogenase Sequence Analysis DNA biology.organism_classification medicine.disease Tropical Diseases Invertebrates Split-Decomposition Method 030104 developmental biology People and Places Africa Microscopy Electron Scanning Sequence Alignment Developmental Biology |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007887 (2019) |
| ISSN: | 1935-2735 |
| Popis: | Myiasis is the infestation of human tissues by dipterous fly larvae of the class Insecta. Clogmia albipunctatus, family Psychodidae, is one of the most medically important insects that cause human myiasis. The aim of the present study is the morphological identification and the molecular characterization of moth flies causing many cases of urinary myiasis in Egypt, based on sequencing of the mitochondrial DNA of the larvae. Seven urinary samples of patients complaining of urinary symptoms and giving a history of low socioeconomic level were examined. Recovered larvae were identified using light microscopy and SEM. For molecular identification, the mitochondrial genes Cytochrome B (cytB), NADH1, NADH1, and 16S were sequenced and phylogenetically analyzed. The morphological and molecular characterization could accurately diagnose our patients to have C. albipunctatus infestation. Such results provided the initial set of data on the molecular identification and phylogenetic analysis of moth flies based on DNA barcoding in Egypt. Author summary Myiasis is an infestation of human tissues and other vertebrates by dipterous fly larvae. Urogenital myiasis is a rare type of myiasis that occurs primarily in poor people with low socioeconomic levels. Accurate diagnosis of this rare form of myiasis is sometimes difficult and requires a detailed study of unfamiliar morphological patterns of the larvae. Based on combined morphological and molecular approaches, our results provided the first set of data on the molecular identification and phylogenetic analysis of moth flies, causing myiasis based on the DNA barcoding in Egypt. Such results present more understanding of causative species epidemiology, the vectorial potentiality, and the proper pest management practices. |
| Databáze: | OpenAIRE |
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