| Autor: |
Yueh-Hsiu Chien, Norman Davidson, Chun-Tsan Deng, Peter M. Chandler |
| Rok vydání: |
1980 |
| Předmět: |
|
| Zdroj: |
Analytical Biochemistry. 102:281-287 |
| ISSN: |
0003-2697 |
| DOI: |
10.1016/0003-2697(80)90153-0 |
| Popis: |
A method for the isolation of segments of any desired length from the 5′ end of retrovirus RNA has been tested. The method is based on selection of 5′-specific segments by hybridizing suitably fragmented genomic (35 S) RNA to mercurated strong stop cDNA followed by chromatography on sulfhydryl-agarose. The method has been shown to be effective for Akv viral RNA by observing the T1 oligonucleotide fingerprints of a 5′-enriched fraction. This fingerprint pattern is of lower complexity than that of total 35 S RNA, contains oligonucleotide spots that have previously been assigned as 5′ specific by conventional fingerprinting methods, and does not overlap with the pattern from 3′-specific RNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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