Induction of goat bone marrow mesenchymal stem cells into putative male germ cells using mRNA for STRA8, BOULE and DAZL
| Autor: | Yixuan Fan, Nie-Hai Tao, Jing Pang, Yongjie Wan, Peizhen Li, Ziyu Wang, Feng Wang, Yanli Zhang, Guomin Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
education.field_of_study Boule Clinical Biochemistry Population Mesenchymal stem cell Biomedical Engineering Bioengineering Synaptonemal complex protein 3 Cell Biology Transfection Biology Andrology 03 medical and health sciences DAZL 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Original Article Stem cell RNA transfection education Biotechnology |
| Zdroj: | Cytotechnology. 71:563-572 |
| ISSN: | 1573-0778 0920-9069 |
| DOI: | 10.1007/s10616-019-00304-7 |
| Popis: | Bone mesenchymal stem cells (BMSCs) have the capacity to differentiate into germ cells (GCs). This study was conducted to develop a non-integrated method of using RNA transfection to derive putative male GCs from goat BMSCs (gBMSCs) in vitro by overexpressing STRA8, BOULE and DAZL. The gBMSCs were induced by co-transfection these three mRNAs together (mi-SBD group) or sequential transfection according to their expression time order in vivo (mi-S + BD group). After transfection, a small population of gBMSCs transdifferentiated into early germ cell-like cells and had the potential to enter meiosis. These cells expressed primordial germ cell specific genes STELLA, C-KIT and MVH, as well as premeiotic genes DAZL, BOULE, STRA8, PIWIL2 and RNF17. Importantly, the expression level of meiotic marker synaptonemal complex protein 3 significantly increased in these transfected two groups compared with control cells by qRT-PCR, immunofluorescence and western blot analysis (P |
| Databáze: | OpenAIRE |
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