Control of brown patch (Rhizoctonia solani) in tall fescue (Festuca arundinacea Schreb.) by host induced gene silencing
| Autor: | Rongda Qu, Ana M. Bailey, Binbin Zhou, Charles L. Niblett |
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| Rok vydání: | 2016 |
| Předmět: |
Festuca
0106 biological sciences 0301 basic medicine Cohesin complex Genes Fungal Brown patch Saccharomyces cerevisiae Plant Science Genetically modified crops Plant disease resistance Rhizoctonia Polymerase Chain Reaction 01 natural sciences Rhizoctonia solani 03 medical and health sciences Databases Genetic Botany Gene Silencing Transgenes RNA Small Interfering Disease Resistance Plant Diseases Genes Essential biology fungi food and beverages General Medicine Blotting Northern Plants Genetically Modified biology.organism_classification Blotting Southern 030104 developmental biology Host-Pathogen Interactions Agronomy and Crop Science Festuca arundinacea 010606 plant biology & botany |
| Zdroj: | Plant Cell Reports. 35:791-802 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-015-1921-7 |
| Popis: | Transgenic tall fescue plants expressing RNAi constructs of essential genes of Rhizoctonia solani were resistant to R. solani. Tall fescue (Festuca arundinacea Schreb.) is an important turf and forage grass species widely used for home lawns and on golf courses in North Carolina and other transition zone states in the US. The most serious and frequently occurring disease of tall fescue is brown patch, caused by a basidiomycete fungus, Rhizoctonia solani. This research demonstrates resistance to brown patch disease achieved by the application of host induced gene silencing. We transformed tall fescue with RNAi constructs of four experimentally determined "essential" genes from R. solani (including genes encoding RNA polymerase, importin beta-1 subunit, Cohesin complex subunit Psm1, and a ubiquitin E3 ligase) to suppress expression of those genes inside the fungus and thus inhibit fungal infection. Four gene constructs were tested, and 19 transgenic plants were obtained, among which 12 plants had detectable accumulation of siRNAs of the target genes. In inoculation tests, six plants displayed significantly improved resistance against R. solani. Lesion size was reduced by as much as 90 %. Plants without RNAi accumulation did not show resistance. To our knowledge, this is the first case that RNAi constructs of pathogen genes introduced into a host plant can confer resistance against a necrotrophic fungus. |
| Databáze: | OpenAIRE |
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