H19 controls reactivation of the imprinted gene network during muscle regeneration
| Autor: | Matthieu Benard, Yann Louault, Paul Monnier, Clémence Martinet, Anne Gabory, Luisa Dandolo |
|---|---|
| Přispěvatelé: | Université Paris Descartes - Paris 5 (UPD5), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Institut Curie [Paris], Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Association Francaise contre les Myopathies (AFM), Agence Nationale de la Recherche [ANR-14-CE11-0022-02], Region Ile-de-France (DIM Biotherapy), Olivier Pourquié |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Satellite Cells Skeletal Muscle Genomic imprinting Mouse [SDV]Life Sciences [q-bio] Mutant Cell Count Myostatin Quiescence Biology Cardiotoxins Muscle hypertrophy Myoblasts 03 medical and health sciences Cardiotoxin Satellite cells medicine Animals Regeneration Gene Regulatory Networks [INFO]Computer Science [cs] Molecular Biology Gene Cell Proliferation Hyperplasia Muscles Skeletal muscle Hypertrophy Molecular biology female genital diseases and pregnancy complications Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure embryonic structures biology.protein RNA Long Noncoding Epigenetics Stem cell Gene Deletion Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2016, 143 (6), pp.962-971. ⟨10.1242/dev.131771⟩ |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.131771 |
| Popis: | International audience; The H19 locus controls fetal growth by regulating expression of several genes from the imprinted gene network (IGN). H19 is fully repressed after birth, except in skeletal muscle. Using loss-of-function H19(Delta 3) mice, we investigated the function of H19 in adult muscle. Mutant muscles display hypertrophy and hyperplasia, with increased Igf2 and decreased myostatin (Mstn) expression. Many imprinted genes are expressed in muscle stem cells or satellite cells. Unexpectedly, the number of satellite cells was reduced by 50% in H19(Delta 3) muscle fibers. This reduction occurred after postnatal day 21, suggesting a link with their entry into quiescence. We investigated the biological function of these mutant satellite cells in vivo using a regeneration assay induced by multiple injections of cardiotoxin. Surprisingly, despite their reduced number, the self-renewal capacity of these cells is fully retained in the absence of H19. In addition, we observed a better regeneration potential of the mutant muscles, with enhanced expression of several IGN genes and genes from the IGF pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|