Topical Application of Angelica sinensis Improves Pruritus and Skin Inflammation in Mice with Atopic Dermatitis-Like Symptoms
| Autor: | Mi Hye Kim, Jae Hong Lee, Eun Hye Kim, Ji Eun Lee, You Yeon Choi, Woong Mo Yang, Jinju Kim, Jae Min Han, Jongki Hong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Angelica sinensis Medicine (miscellaneous) Inflammation Substance P Immunoglobulin E Dermatitis Atopic Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Humans Antipruritic Interleukin 4 Skin Mice Inbred BALB C Nutrition and Dietetics biology Interleukin-6 Plant Extracts Tumor Necrosis Factor-alpha business.industry Pruritus NF-kappa B Atopic dermatitis medicine.disease biology.organism_classification 030104 developmental biology chemistry 030220 oncology & carcinogenesis Immunology biology.protein Female Tumor necrosis factor alpha Interleukin-4 medicine.symptom business medicine.drug |
| Zdroj: | Journal of Medicinal Food. 19:98-105 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2015.3489 |
| Popis: | Angelica sinensis (AS) is one of the most popular medicinal foods used as a hematopoietic herb and also traditionally applied topically for skin disorders. However, the effectiveness of AS on atopic dermatitis (AD) has not been reported yet. This study was conducted to evaluate the antipruritic and anti-inflammatory effects of AS on regulating AD-related mediators in DNCB (2,4-dinitrochlorobenzene)-induced mice. AS was topically applied to the dorsal skin of DNCB-challenged mice for 11 days. Alteration of skin thickness was measured for assessment of histological improvement. In addition, the number of mast cells, the level of serum immunoglobulin E (IgE), the counting of scratching behavior, and the expression of substance P were evaluated. Also, the expressions of cytokines, nuclear factor κB (NF-κB), phospho-IκBα, and mitogen-activated protein kinases (MAPKs) were measured for evaluating the improvement of skin inflammation. The repeated treatment of AS significantly inhibited the skin thickness, the number of mast cells, and the level of serum IgE. Moreover, AS significantly suppressed the increased scratching behavior and the expression of substance P compared to the DNCB group. Topical application of AS also reduced the level of cytokines (IL-4, IL-6, TNF-α, and IFN-γ) as well as the expressions of NF-κB, phospho-IκBα, and phospho-MAPKs in the dorsal skin. The results of our study suggest that topical application of AS might have efficacy for modulating pruritus and inflammation in AD. Further studies are required to further characterize the mechanism of actions of AS. |
| Databáze: | OpenAIRE |
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